Anatomical substrates of Rapid Eye Movement sleep rebound in a rodent model of post-sevoflurane sleep disruption

Author:

Atluri Navya1,Dulko Elzbieta2,Jedrusiak Michal1,Klos Joanna3ORCID,Osuru Hari P1,Davis Eric4,Beenhakker Mark5,Kapur Jaideep6,Zuo Zhiyi1,Lunardi Nadia1ORCID

Affiliation:

1. 1Department of Anesthesiology, University of Virginia, Charlottesville, VA, USA

2. 2Neuroscience Graduate Program, University of Virginia, Charlottesville, VA, USA

3. 3Max Planck Institute for Biological Intelligence, Munich, Germany

4. 4Department of Internal Medicine, University of Virginia, Charlottesville, VA, USA

5. 5Department of Pharmacology, University of Virginia, Charlottesville, VA, USA

6. 6Department of Neurology, University of Virginia, Charlottesville, VA, USA

Abstract

Background Previous research suggests that sevoflurane anesthesia may prevent the brain from accessing REM sleep. If true, then patterns of neural activity observed in REM-on and REM-off neuronal populations during recovery from sevoflurane should resemble those seen after REM sleep deprivation. In this study we hypothesized that, relative to controls, animals exposed to sevoflurane present with a distinct expression pattern of c-Fos, a marker of neuronal activation, in a cluster of nuclei classically associated with REM sleep, and that such expression in sevoflurane -exposed and REM sleep-deprived animals is largely similar. Methods Adult rats and Targeted Recombination in Active Populations mice were implanted with electroencephalographic electrodes for sleep-wake recording and randomized to sevoflurane, REM deprivation or control conditions. Conventional c-Fos immunohistochemistry and genetically-tagged c-Fos labeling were used to quantify activated neurons in a group of REM-associated nuclei in the midbrain and basal forebrain. Results REM sleep duration increased during recovery from sevoflurane anesthesia relative to controls (157.0 ± 24.8 min vs 124.2 ± 27.8 min, P=0.003), and temporally correlated with increased c-Fos expression in the sublaterodorsal nucleus (SLD), a region active during REM sleep (176.0 ± 36.6 cells vs 58.8 ± 8.7, P=0.014), and decreased c-Fos expression in the ventrolateral periaqueductal gray (vlPAG), a region that is inactive during REM sleep (34.8 ± 5.3 cells vs 136.2 ± 19.6, P=0.001). Fos changes similar to those seen in sevoflurane-exposed mice were observed in REM-deprived animals, relative to controls (SLD: 85.0 ± 15.5 cells vs 23.0 ± 1.2, P=0.004; vlPAG: 652.8 ± 71.7 cells vs 889.3 ± 66.8, P=0.042). Conclusions In rodents recovering from sevoflurane, REM-on and REM-off neuronal activity maps closely resemble those of REM sleep-deprived animals. These findings provide new evidence in support of the idea that sevoflurane does not substitute for endogenous REM sleep.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Anesthesiology and Pain Medicine

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3