Multicompartmental traumatic injury induces sex-specific alterations in the gut microbiome

Author:

Munley Jennifer A.,Kelly Lauren S.,Park Gwoncheol,Gillies Gwendolyn S.,Pons Erick E.,Kannan Kolenkode B.,Whitley Elizabeth M.,Bible Letitia E.,Efron Philip A.,Nagpal Ravinder,Mohr Alicia M.

Abstract

BACKGROUND Previous preclinical studies have demonstrated an altered gut microbiome after traumatic injury; however, the impact of sex on dysbiosis remains unknown. We hypothesized that the “pathobiome” phenotype induced by multicompartmental injuries and chronic stress is host sex specific with unique microbiome signatures. METHODS Male and proestrus female Sprague-Dawley rats (n = 8/group) aged 9 weeks to 11 weeks were subjected to either multicompartmental injury (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofractures), PT plus 2 hours daily chronic restraint stress (PT/CS) or naive controls. Fecal microbiome was measured on Days 0 and 2 using high-throughput 16S rRNA sequencing and Quantitative Insights Into Microbial Ecology bioinformatics analyses. Microbial alpha-diversity was assessed using Chao1 (number of different unique species) and Shannon (species richness and evenness) indices. Beta-diversity was assessed using principle coordinate analysis. Intestinal permeability was evaluated by plasma occludin and lipopolysaccharide binding protein. Histologic evaluation of ileum and colon tissues was scored for injury by a blinded pathologist. Analyses were performed in GraphPad and R, with significance defined as p < 0.05 between males versus females. RESULTS At baseline, females had significantly elevated alpha-diversity (Chao1, Shannon indices) compared with males (p < 0.05) which was no longer present 2 days postinjury in PT and PT/CS. Beta-diversity also differed significantly between males and females after PT (p = 0.01). At Day 2, the microbial composition in PT/CS females was dominated by Bifidobacterium, whereas PT males demonstrated elevated levels of Roseburia (p < 0.01). The PT/CS males had significantly elevated ileum injury scores compared with females (p = 0.0002). Plasma occludin was higher in PT males compared with females (p = 0.004); plasma lipopolysaccharide binding protein was elevated in PT/CS males (p = 0.03). CONCLUSION Multicompartmental trauma induces significant alterations in microbiome diversity and taxa, but these signatures differ by host sex. These findings suggest that sex is an important biological variable that may influence outcomes after severe trauma and critical illness.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Critical Care and Intensive Care Medicine,Surgery

Reference47 articles.

1. Sepsis and the microbiome: a vicious cycle;J Infect Dis,2021

2. Collapse of the microbiome, emergence of the pathobiome, and the immunopathology of Sepsis;Crit Care Med,2017

3. Pathophysiology of the gut and the microbiome in the host response;Pediatr Crit Care Med,2017

4. The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton;J Biol Chem,1998

5. Plasma markers of disrupted gut permeability in severe COVID-19 patients;Front Immunol,2021

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