Affiliation:
1. Department of Nuclear Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China
Abstract
Objective
To prepare a single-photon molecular probe easily labeled with 99mTc for evaluating the expression status of the human epidermal growth factor receptor 2 (HER2) receptor in ovarian cancer.
Materials and methods
The HEHEHE tag was added to the amino terminus of the affibody ZHER2:V2 by the method of gene recombinant expression, and a new affibody was synthesized which was easy to be labeled with 99mTc. The newly prepared affibody was labeled with 99mTc, and pharmacokinetic studies were carried out.
Results
A new affibody (HE)3ZHER2:V2 was prepared by the method of gene recombination and expression, which is easy to be labeled with 99mTc. The 99mTc labeling of the affibody can reach about 95% at 90°C. The pharmacokinetic study has shown that the 99mTc-labeled molecular probe has a fast clearance time in the blood and little side effect, which may be a promising single-photon emission computed tomography (SPECT) imaging agent.
Conclusion
The affibody (HE)3ZHER2:V2 is easy to be labeled with 99mTc, has a high yield and has a suitable half-life in vivo, which is suitable for the next step in ovarian cancer model imaging research.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Subject
Radiology, Nuclear Medicine and imaging,General Medicine
Cited by
1 articles.
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