PYCR1, BANF1, and STARD8 Expression in Gastric Carcinoma: A Clinicopathologic, Prognostic, and Immunohistochemical Study

Author:

Harb Ola A.1,Elfeky Mariem A.1,Alabiad Mohamed Ali1,Hemeda Rehab2,Allam Ahmed S.3,El Hawary Amr T.3,Elbaz Mohammed4,Sharaf Ahmed L.5,Gertallah Loay M.6,Abdelaziz Ahmed Mesbah6,Shalaby Amany Mohamed7,Alorini Mohammed8,Yahia Amar Ibrahim Omer910,Negm Mohamed6

Affiliation:

1. Basic Medical Sciences

2. Clinical Oncology and Nuclear Medicine

3. Internal Medicine

4. Department of Internal Medicine, Faculty of Medicine, Cairo University, Cairo

5. Tropical Medicine, Faculty of Medicine, Zagazig University, Zagazig

6. General Surgery

7. Department of Histology and Cell Biology, Faculty of Medicine, Tanta University, Tanta

8. Department of Basic Medical Sciences, Unaizah College of Medicine and Medical Sciences, Qassim University, Unaizah

9. Department of Pathology, College of Medicine, University of Bisha, Bisha, Kingdom of Saudi Arabia

10. Department of Pathology, Faculty of Medicine and Health Sciences, University of Kordofan, Elobeid, Sudan

Abstract

Background: It will be important to understand the molecular pathways of gastric cancer (GC) occurrence and progression, thus detecting predictive and prognostic biomarkers of GC. Pyrroline-5-carboxylate reductase 1 (PYCR1) was upregulated in many cancers, suggesting its possible roles in carcinogenesis and tumor metastases. Barrier-of-autointegration factor 1 (BANF1) is a protein family that plays essential roles in maintaining the integrity of an intact cellular genome. Rho-GTPs are molecular switches that control many signal transduction pathways in normal cells, including 3 subgroups from 1 to 3 (DLC1-3). DLC-3, known as StAR-related lipid transfer domain protein 8 (STARD8), and its role in cancers were not sufficiently studied. The study aimed to investigate the significance of PYCR1, BANF1, and STARD8 protein expression in GC tissues and normal gastric mucosa retrieved from patients with GC to detect prognostic roles of expression. Patients and Methods: Specimens were collected from 100 patients with gastric carcinoma. After the application of the inclusion criteria of the study, we prepared 100 paraffin blocks from samples of the 100 included patients; each block included samples from gastric carcinoma and adjacent non-neoplastic gastric mucosa. We assessed the expression of PYCR1, BANF1, and STARD8 using immunohistochemistry in all studied samples. We followed patients for the detection of disease progression and survival rates. We correlate PYCR1, BANF1, and STARD8 expression with clinical, pathologic, and prognostic parameters. Results: Overexpression of PYCR1 and BANF1 and decreased expression of STARD8 was found in gastric carcinoma tissues than adjacent non-neoplastic gastric mucosa (P<0.001), and was positively associated with high grade (P=0.006), depth of tumor invasion, presence of lymph nodes metastases and advanced stage (P=0.001), high incidence of GC progression, recurrence, unfavorable disease-free survival (P=0.003) and unfavorable overall survival rates (P<0.001). Thus, it was revealed that; in univariate and multivariate analyses, levels of PYCR1, BANF1, and STARD8 are associated with the overall survival rate of GC patients. Conclusions: We showed that overexpression of PYCR1 and BANF1 and decreased expression of STARD8 in GC tissues was associated with poor prognosis and GC progression.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Medical Laboratory Technology,Histology,Pathology and Forensic Medicine

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