The Intestinal Tract Brush Border in Young Children Uniformly Expresses Guanylate Cyclase C

Author:

Di Guglielmo Matthew D.1,Holbrook Jennifer2,Stabley Deborah2,Robbins Katherine M.2,Boyce Bobbie2,Hardy Heather2,Adeyemi Adebowale1

Affiliation:

1. Division of Gastroenterology, Department of Pediatrics

2. Nemours Biomedical Research, Nemours Children’s Health, Nemours Children’s Hospital, Delaware Valley, DE

Abstract

The present study examined staining of guanylate cyclase C (GCC/GUCY2C) in the small and large intestines of children younger than age 7 years. Normal intestinal tissue from children aged 0 to 7 years was stained using GCC, uroguanylin, and villin antibodies and scored for staining intensity. A subset underwent quantitative real-time polymerase chain reaction. Data were analyzed using t test of independent means, descriptive statistics, and logistic regression. Four hundred sixty-four specimens underwent immunohistochemistry; 291 specimens underwent real-time polymerase chain reaction. GCC, villin, and uroguanylin were detected across age groups and anatomic sites. No significant differences were identifiable across age groups. GUCY2C and uroguanylin mRNA was detected in all samples, with no variability of statistical significance of either target-to-villin normalization between any age cohorts. A gradient of expression of GCC across age groups does not seem to exist.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Medical Laboratory Technology,Histology,Pathology and Forensic Medicine

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