Glutamic acid decarboxylase immunoreactivity in the olfactory bulb of a reptile

Abstract

The objective is to determine the distribution of glutamic acid decarboxylase (GAD) in the olfactory bulb of a crocodilian, Caiman crocodilus. Avidin-biotin immunohistochemical methodology using a polyclonal antibody to GAD raised in sheep was employed. The following controls were used: substitution of the primary antibody with preimmune sheep serum at concentrations equal to that of the primary antibody; omission of the primary antibody; and omission of the primary antibody and biotinylated rabbit antisheep immunoglobulin. No GAD (+) cells were observed in the control sections. Based on cell and fiber staining, the layering and neuronal organization of the olfactory bulb in Caiman were similar to other vertebrates, including other reptiles. The following elements were GAD (+): granule cells, certain neurons in the outer plexiform layer, periglomerular neurons, and the glomeruli themselves. GAD (+) puncta were present throughout the olfactory bulb. In conclusion, these results in Caiman were similar, in part, to comparable studies in mammals and birds. Taken together, these data indicate that crocodiles not only have a similar pattern of layers that other amniotes possess but also that the immunocytochemical signatures of certain elements of the olfactory bulb are likewise shared.