Affiliation:
1. Department of Dermatology, Medical Faculty, University Hospital Duesseldorf
2. Dermatologie am Luegplatz
3. Faculty of Health, University Witten/Herdecke.
Abstract
Background:
Hyaluronidase (HYAL) is regarded as the standard for the management of complications associated with hyaluronan (HA)-based fillers. Therefore, the understanding of interactions of HA fillers and HYAL is essential.
Methods:
Nine different commercially available HA fillers (Belotero, Juvéderm, and Restylane) with varying degrees of cross-linking were used for the analysis. Fluorescently dyed HA fillers were individually incubated with varying doses of HYAL [bovine HYAL (Hylase “Dessau”; Riemser Pharma, Germany); 5, 10, and 20 U/mL] or sodium chloride and monitored by time-lapse microscopy. HA filler degradation was assessed as a decrease in fluorescence intensity of HA filler plus HYAL compared to HA filler plus control, quantified by computerized image analysis.
Results:
HA fillers show significant differences in their reaction to HYAL. Levels of degradation of HA fillers are positively correlated with increasing concentrations of HYAL. At the highest concentration of HYAL (20 U/mL), all fillers except one (Belotero Volume) reached a significant level of degradation at 5 to 9 hours.
Conclusions:
In this study, the authors show that most HA fillers can be dissolved by HYAL in a dose- and time-dependent manner. Of note, the fillers’ technology and degree of cross-linking seem to exert stronger effects on the degradability by HYAL as compared to the concentration of HA.
Clinical Relevance Statement:
The authors' in vitro analyses support clinical recommendations stating that in the case of a vascular filler incident, HYAL should be applied early and at significant doses ("Time is skin!").
CLINICAL QUESTION/LEVEL OF EVIDENCE:
Therapeutic, V.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Cited by
2 articles.
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