Patient-derived bladder cancer organoid model to predict sensitivity and feasibility of tailored precision therapy

Author:

Jiang Ying1,Sun Xun2,Song Xiaoyun1,Li Zhen1,Zhang Ping1,Zhang Wen1,Tang Dongqi

Affiliation:

1. Center for Gene and Immunotherapy, The Second Hospital of Shandong University, Cheeloo College of Medicine, Jinan, China

2. Department of Urology, Shandong Provincial Hospital, Shandong university, Shandong First Medical University, Jinan, China

Abstract

Abstract Background Bladder cancer is a common and highly heterogeneous malignant tumor with a relatively poor prognosis. Thus, personalized treatment strategies for bladder cancer are essential for improving patient outcomes. Materials and methods We developed an efficient 3-dimensional in vitro organoid culture system for bladder cancer organoids (BCOs), which maintains the homology with the original patient tumors and the heterogeneity between different individuals. In addition, we constructed chimeric antigen receptor (CAR)-T cells targeting B7H3 and evaluated the antitumor function of CAR-T cells by coculturing them with BCOs. Results The BCOs closely resembled the characteristics of human tumors and were used to test individual sensitivity to platinum-based drugs and olaparib therapy. Coculture with CAR-T cells demonstrated specific antigen recognition and immune activation, indicating their potential in immunotherapy. Conclusions Our study highlights the potential of BCOs to facilitate the development of personalized medicine for bladder cancer and improve the efficiency of drug discovery for bladder cancer therapy.

Publisher

Ovid Technologies (Wolters Kluwer Health)

Subject

Urology,Oncology,Reproductive Medicine

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