CRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice

Author:

Wang Chih-Hao12ORCID,Lundh Morten134ORCID,Fu Accalia56ORCID,Kriszt Rókus78,Huang Tian Lian1ORCID,Lynes Matthew D.1ORCID,Leiria Luiz O.910ORCID,Shamsi Farnaz1ORCID,Darcy Justin1,Greenwood Bennett P.11,Narain Niven R.11ORCID,Tolstikov Vladimir11ORCID,Smith Kyle L.12ORCID,Emanuelli Brice3ORCID,Chang Young-Tae1314ORCID,Hagen Susan12,Danial Nika N.5ORCID,Kiebish Michael A.11ORCID,Tseng Yu-Hua115ORCID

Affiliation:

1. Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.

2. Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan.

3. Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, DK-2200, Denmark.

4. Gubra Aps, Hørsholm, DK-2970, Denmark.

5. Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA.

6. Department of Cell Biology, Harvard Medical School, Boston, MA 02215, USA.

7. Department of Biomedical Engineering, National University of Singapore, Singapore, 117583.

8. Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, Singapore 119077, Singapore.

9. Department of Pharmacology, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, 14049-900, Brazil.

10. Center of Research of Inflammatory Diseases, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, 14049-900, Brazil.

11. BERG, Framingham, MA 01701, USA.

12. Department of Surgery, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.

13. Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 34126, Republic of Korea.

14. Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.

15. Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.

Abstract

Human white adipocytes engineered to express UCP1 activate endogenous BAT and protect against diet-induced obesity when transplanted into mice.

Funder

NIH Office of the Director

Medical Research and Materiel Command, U.S. Army Medical Department

Danish Agency for Science, Technology and Innovation

Novo Nordisk Foundation Center for Basic Metabolic Research

ADA Foundation

JDRF

Ministry of Science and Technology, Taiwan

Sao Paulo Research Foundation

American Heart Association

Publisher

American Association for the Advancement of Science (AAAS)

Subject

General Medicine

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