Rapid detection of Mycobacterium tuberculosis in sputum with a solvatochromic trehalose probe

Author:

Kamariza Mireille1ORCID,Shieh Peyton2ORCID,Ealand Christopher S.3,Peters Julian S.3ORCID,Chu Brian2ORCID,Rodriguez-Rivera Frances P.2,Babu Sait Mohammed R.4ORCID,Treuren William V.5,Martinson Neil36,Kalscheuer Rainer4ORCID,Kana Bavesh D.37ORCID,Bertozzi Carolyn R.28ORCID

Affiliation:

1. Department of Biology, Stanford University, Stanford, CA 94305, USA.

2. Department of Chemistry, Stanford University, Stanford, CA 94305, USA.

3. Department of Science and Technology/National Research Foundation Centre of Excellence for Biomedical Tuberculosis Research, Faculty of Health Sciences, University of Witwatersrand, National Health Laboratory Service, Johannesburg, South Africa.

4. Institute of Pharmaceutical Biology and Biotechnology, Heinrich Heine University Duesseldorf, Universitätsstrasse 1, 40225 Duesseldorf, Germany.

5. Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA.

6. Perinatal HIV Research Unit (PHRU), SA MRC Soweto Matlosana Collaborating Centre for HIV/AIDS and TB, University of the Witwatersrand, Johannesburg, South Africa.

7. Medical Research Council–Centre for the AIDS Programme of Research in South Africa (CAPRISA) HIV-TB Pathogenesis and Treatment Research Unit, Durban, South Africa.

8. Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.

Abstract

A solvatochromic trehalose dye conjugate allows the fast and specific detection of Mycobacterium tuberculosis in sputum samples.

Funder

National Institutes of Health

Bill and Melinda Gates Foundation

Publisher

American Association for the Advancement of Science (AAAS)

Subject

General Medicine

Reference46 articles.

1. World Health Organization (WHO) “Global tuberculosis report 2016” (WHO Press 2016); http://apps.who.int/iris/bitstream/10665/250441/1/9789241565394-eng.pdf.

2. World Health Organization (WHO) “High-priority target product profiles for new tuberculosis diagnostics: Report of a consensus meeting” (WHO Press 2014); http://apps.who.int/iris/bitstream/10665/135617/1/WHO_HTM_TB_2014.18_eng.pdf.

3. World Health Organization (WHO) “Molecular line probe assays for rapid screening of patients at risk of multidrug-resistant tuberculosis (MDR-TB)” (WHO Press 2008)” www.who.int/tb/features_archive/policy_statement.pdf.

4. Laboratory Diagnosis of Tuberculosis in Resource-Poor Countries: Challenges and Opportunities

5. Zur Färbung des Tuberkelbacillus

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