A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID-19 convalescent plasma

Author:

Fenwick Craig1ORCID,Turelli Priscilla2ORCID,Pellaton Céline1ORCID,Farina Alex1,Campos Jérémy1,Raclot Charlène2ORCID,Pojer Florence2ORCID,Cagno Valeria34ORCID,Nusslé Semira Gonseth5ORCID,D’Acremont Valerie56,Fehr Jan7,Puhan Milo7,Pantaleo Giuseppe189ORCID,Trono Didier2ORCID

Affiliation:

1. Service of Immunology and Allergy, Department of Medicine, Lausanne University Hospital, University of Lausanne, Lausanne 1011, Switzerland.

2. School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland.

3. Department of Microbiology and Molecular Medicine, University of Geneva, Geneva 1211, Switzerland.

4. Institute of Microbiology, Lausanne University Hospital, University of Lausanne, Lausanne 1011, Switzerland.

5. Centre for Primary Care and Public Health, University of Lausanne, Lausanne 1011, Switzerland.

6. Swiss Tropical and Public Health Institute, University of Basel, Basel 4001, Switzerland.

7. Epidemiology, Biostatistics and Prevention Institute, University of Zurich, Zurich 8001, Switzerland.

8. Swiss Vaccine Research Institute, Lausanne University Hospital, University of Lausanne, Lausanne 1011, Switzerland.

9. VRI, Université Paris-Est Créteil, Faculté de Médicine, INSERM U955, Créteil 94010, France.

Abstract

A multiplexed virus-free surrogate neutralization assay measures antibody responses against spike protein mutations in SARS-CoV-2 variants.

Funder

Center for Global Health

École Polytechnique Fédérale de Lausanne

Swiss National Science Foundation

Fondation Leenaards

Université de Lausanne

Centre Hospitalier Universitaire Vaudois

Swiss Vaccine Research Institute

EPFL COVID

Horizon 2020 research and innovation program

Publisher

American Association for the Advancement of Science (AAAS)

Subject

General Medicine

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