Early sample tagging and pooling enables simultaneous SARS-CoV-2 detection and variant sequencing

Author:

Chappleboim Alon12ORCID,Joseph-Strauss Daphna12ORCID,Rahat Ayelet12,Sharkia Israa12,Adam Miriam3,Kitsberg Daniel3,Fialkoff Gavriel12,Lotem Matan12,Gershon Omer12ORCID,Schmidtner Anna-Kristina3,Oiknine-Djian Esther45,Klochendler Agnes6ORCID,Sadeh Ronen12ORCID,Dor Yuval6ORCID,Wolf Dana45ORCID,Habib Naomi3ORCID,Friedman Nir12ORCID

Affiliation:

1. Alexander Silberman Institute of Life Science, Hebrew University of Jerusalem, Jerusalem 9190401, Israel.

2. Rachel and Selim Benin School of Computer Science, Hebrew University of Jerusalem, Jerusalem 9190401, Israel.

3. Edmond and Lily Safra Center for Brain Sciences, Hebrew University of Jerusalem, Jerusalem 9190401, Israel.

4. Hadassah Hebrew University Medical Center, Jerusalem 9112001, Israel.

5. Lautenberg Centre for Immunology and Cancer Research, IMRIC, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9112001, Israel.

6. Department of Developmental Biology and Cancer Research, IMRIC, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9112001, Israel.

Abstract

A sequencing-based detection and genotyping assay for SARS-CoV-2 is based on early sample pooling using barcoded oligo hybridization.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

General Medicine

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