Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum

Author:

Bosch Irene12ORCID,de Puig Helena13ORCID,Hiley Megan1,Carré-Camps Marc14ORCID,Perdomo-Celis Federico5ORCID,Narváez Carlos F.5,Salgado Doris M.5,Senthoor Dewahar1ORCID,O’Grady Madeline1,Phillips Elizabeth1,Durbin Ann16ORCID,Fandos Diana14,Miyazaki Hikaru1,Yen Chun-Wan1,Gélvez-Ramírez Margarita7ORCID,Warke Rajas V.8,Ribeiro Lucas S.9ORCID,Teixeira Mauro M.9ORCID,Almeida Roque P.10ORCID,Muñóz-Medina José E.11ORCID,Ludert Juan E.12,Nogueira Mauricio L.13,Colombo Tatiana E.13,Terzian Ana C. B.13,Bozza Patricia T.14ORCID,Calheiros Andrea S.14,Vieira Yasmine R.15,Barbosa-Lima Giselle15,Vizzoni Alexandre15,Cerbino-Neto José15,Bozza Fernando A.1516,Souza Thiago M. L.1417,Trugilho Monique R. O.18ORCID,de Filippis Ana M. B.19,de Sequeira Patricia C.19ORCID,Marques Ernesto T. A.2021ORCID,Magalhaes Tereza2022,Díaz Francisco J.23ORCID,Restrepo Berta N.24,Marín Katerine24,Mattar Salim25ORCID,Olson Daniel26,Asturias Edwin J.26,Lucera Mark27,Singla Mohit28,Medigeshi Guruprasad R.29ORCID,de Bosch Norma30,Tam Justina131ORCID,Gómez-Márquez Jose1ORCID,Clavet Charles31,Villar Luis7ORCID,Hamad-Schifferli Kimberly332ORCID,Gehrke Lee133ORCID

Affiliation:

1. Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

2. Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.

3. Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

4. Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain.

5. Programa de Medicina, Facultad de Salud, Universidad Surcolombiana, Neiva, Colombia.

6. Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, MA 02115, USA.

7. Universidad Industrial de Santander and AEDES Program (Alianza para el desarrollo de estrategias que disminuyan el impacto de enfermedades transmitidas por Aedes como resultado del estudio de sus endemias y epidemias), Bucaramanga, Santander, Colombia.

8. HiMedia Laboratories Pvt. Ltd., Mumbai, India.

9. Immunopharmacology Group, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Belo Horizonte, Brazil.

10. Departamento de Medicina Interna e Patologia, Hospital Universitário/Empresa Brasileira de Serviços Hospitalares (EBSERH), Universidade Federal de Sergipe, Aracaju, Brazil.

11. Laboratorio Central de Epidemiología, Instituto Mexicano del Seguro Social, Avenida Jacarandas S/N, Esquina Circuito Interior, Colonia La Raza Del Azcapotzalco, Código Postal 02990 México D.F., México.

12. Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México, México.

13. Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, Brazil.

14. Immunopharmacology Laboratory, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil.

15. National Institute of Infectious Disease Evandro Chagas, FIOCRUZ, Rio de Janeiro, Brazil.

16. D’Or Institute of Research and Education (IDOR), Rio de Janeiro, Brazil.

17. National Institute for Science and Technology on Innovation on Neglected Diseases (INCT/IDN), Center for Technological Development in Health (CDTS), FIOCRUZ, Rio de Janeiro, Brazil.

18. Toxinology Laboratory and Center for Technological Development in Health (CDTS), FIOCRUZ, Rio de Janeiro, Brazil.

19. Flavivirus Laboratory, FIOCRUZ, Rio de Janeiro, Brazil.

20. Aggeu Magalhães Research Center, FIOCRUZ, Pernambuco, Recife, Brazil.

21. Department of Infectious Disease and Microbiology, University of Pittsburgh, Pittsburgh, PA 15213, USA.

22. Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.

23. Immunovirology Group, School of Medicine, University of Antioquia, Medellín, Colombia.

24. Instituto Colombiano de Medicina Tropical (ICMT), Universidad CES, Sabaneta, Antioquia, Colombia.

25. Universidad de Córdoba, Montería, Córdoba, Colombia.

26. Division of Infectious Diseases, Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO 80045, USA.

27. Division of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Aurora, CO 80045, USA.

28. Department of Paediatrics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.

29. Translational Health Science and Technology Institute, Faridabad, India.

30. Universidad Central de Venezuela, Caracas, Venezuela.

31. Winchester Engineering Analytical Center (WEAC), Winchester, MA 01890, USA.

32. Department of Engineering, University of Massachusetts Boston, Boston, MA 02125, USA.

33. Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA.

Abstract

A low-cost, equipment-free rapid antigen test distinguishes dengue virus serotypes and Zika virus in patient sera without detectable cross-reactivity.

Funder

National Institutes of Health

Sao Paolo Research Foundation FAPESP

The Science, Technology and Innovation Fund of Colombia

The AEDES Network

Marc and Lynn Benioff

ORISE Fellowship

La Caixa Fellowship

Rafael del Pino Fellowship

Broshy Fellowship

MIT-Tata Center Fellowship

MIT-SUTD Fellowship

Publisher

American Association for the Advancement of Science (AAAS)

Subject

General Medicine

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