An engineered human albumin enhances half-life and transmucosal delivery when fused to protein-based biologics

Author:

Bern Malin12ORCID,Nilsen Jeannette12ORCID,Ferrarese Mattia3ORCID,Sand Kine M. K.124ORCID,Gjølberg Torleif T.125,Lode Heidrun E.125ORCID,Davidson Robert J.6,Camire Rodney M.67,Bækkevold Espen S.8,Foss Stian12ORCID,Grevys Algirdas12ORCID,Dalhus Bjørn9ORCID,Wilson John10,Høydahl Lene S.111ORCID,Christianson Gregory J.10ORCID,Roopenian Derry C.10ORCID,Schlothauer Tilman12,Michaelsen Terje E.1314ORCID,Moe Morten C.5ORCID,Lombardi Silvia3ORCID,Pinotti Mirko3ORCID,Sandlie Inger14,Branchini Alessio3ORCID,Andersen Jan Terje12ORCID

Affiliation:

1. Centre for Immune Regulation (CIR) and Department of Immunology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.

2. Institute of Clinical Medicine and Department of Pharmacology, University of Oslo and Oslo University Hospital, 0372 Oslo, Norway.

3. Department of Life Sciences and Biotechnology and LTTA, University of Ferrara, 44121 Ferrara, Italy.

4. CIR and Department of Biosciences, University of Oslo, 0371 Oslo, Norway.

5. Department of Ophthalmology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0450 Oslo, Norway.

6. The Children’s Hospital of Philadelphia, The Raymond G. Perelman Center for Cellular and Molecular Therapeutics, Philadelphia, PA 19104, USA.

7. Department of Pediatrics, Division of Hematology, University of Pennsylvania, Philadelphia, PA 19104, USA.

8. CIR and Department of Pathology, University of Oslo and Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway.

9. Department for Medical Biochemistry, Institute for Clinical Medicine and Department for Microbiology, Clinic for Laboratory Medicine, University of Oslo, 0372 Oslo, Norway.

10. The Jackson Laboratory, Bar Harbor, ME 04609, USA.

11. KG Jebsen Coeliac Disease Research Centre, University of Oslo, 0372 Oslo, Norway.

12. Biochemical and Analytical Research, Large Molecule Research, Roche Pharma Research and Early Development (pRED), Roche Innovation Center Munich, 82377 Penzberg, Germany.

13. Department of Infectious Disease Immunology, Norwegian Institute of Public Health, 0456 Oslo, Norway.

14. Department of Chemical Pharmacy, School of Pharmacy, University of Oslo, 0371 Oslo, Norway.

Abstract

The human albumin variant E505Q/T527M/K573P improves pharmacokinetics of protein-based drugs and facilitates transport across mucosal epithelia.

Funder

South-Eastern Norway Regional Health Authority

The Research Council of Norway

The Research Council of Norway through its program for Global Health and Vaccination Research

The South-Eastern Norway Regional Health Authority

PFIZER EuroAspire projects

Bayer Early Career Investigator Award (Bayer Hemophilia Award Program) 2018

Publisher

American Association for the Advancement of Science (AAAS)

Subject

General Medicine

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