2.4-Å structure of the double-ring <i>Gemmatimonas phototrophica</i> photosystem-Reference-Cited by-同舟云学术

2.4-Å structure of the double-ring Gemmatimonas phototrophica photosystem

Published:2022-02-18 Issue:7 Volume:8 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Qian Pu¹²^ORCID,Gardiner Alastair T.³^ORCID,Šímová Ivana⁴^ORCID,Naydenova Katerina⁵^ORCID,Croll Tristan I.⁶^ORCID,Jackson Philip J.²^ORCID,Nupur ³^ORCID,Kloz Miroslav⁷,Čubáková Petra⁷^ORCID,Kuzma Marek⁸,Zeng Yonghui⁹,Castro-Hartmann Pablo¹^ORCID,van Knippenberg Bart¹^ORCID,Goldie Kenneth N.¹⁰^ORCID,Kaftan David³^ORCID,Hrouzek Pavel³^ORCID,Hájek Jan³,Agirre Jon¹¹^ORCID,Siebert C. Alistair¹²^ORCID,Bína David⁴^ORCID,Sader Kasim¹^ORCID,Stahlberg Henning¹³^ORCID,Sobotka Roman³⁴^ORCID,Russo Christopher J.⁵^ORCID,Polívka Tomáš⁴^ORCID,Hunter C. Neil²^ORCID,Koblížek Michal³^ORCID

Affiliation:

1. Materials and Structural Analysis, Thermo Fisher Scientific, Achtseweg Noord 5, 5651 GG Eindhoven, Netherlands.

2. School of Biosciences, University of Sheffield, Sheffield S10 2TN, UK.

3. Center Algatech, Institute of Microbiology, Czech Academy of Sciences, 37981 Třeboň, Czechia.

4. Faculty of Science, University of South Bohemia, 37005 České Budějovice, Czechia.

5. MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.

6. Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK.

7. ELI Beamlines, Institute of Physics of the Czech Academy of Sciences, Na Slovance 1999/2, 182 21 Prague, Czechia.

8. Lab of Molecular Structure, Institute of Microbiology, Czech Academy of Sciences, Prague, Czechia.

9. Department of Plant and Environmental Sciences, University of Copenhagen, Nørregade 10, DK-1165 Copenhagen, Denmark.

10. BioEM lab, Biozentrum, University of Basel, Mattenstrasse 26, 4058 Basel, Switzerland.

11. Department of Chemistry, University of York, York YO10 5DD, UK.

12. Electron Bio-imaging Centre, Diamond Light Source, Didcot OX11 0DE, UK.

13. Laboratory of Biological Electron Microscopy, Institute of Physics, SB, EPFL, and Faculty of Biology and Medicine, Uni Lausanne, CH-1015 Lausanne, Switzerland.

Abstract

Phototrophic Gemmatimonadetes evolved the ability to use solar energy following horizontal transfer of photosynthesis-related genes from an ancient phototrophic proteobacterium. The electron cryo-microscopy structure of the Gemmatimonas phototrophica photosystem at 2.4 Å reveals a unique, double-ring complex. Two unique membrane-extrinsic polypeptides, RC-S and RC-U, hold the central type 2 reaction center (RC) within an inner 16-subunit light-harvesting 1 (LH1) ring, which is encircled by an outer 24-subunit antenna ring (LHh) that adds light-gathering capacity. Femtosecond kinetics reveal the flow of energy within the RC-dLH complex, from the outer LHh ring to LH1 and then to the RC. This structural and functional study shows that G. phototrophica has independently evolved its own compact, robust, and highly effective architecture for harvesting and trapping solar energy.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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