A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9-Reference-Cited by-同舟云学术

A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9

Published:2020-07-17 Issue:29 Volume:6 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Collias D.¹^ORCID,Leenay R. T.¹,Slotkowski R. A.¹^ORCID,Zuo Z.²³,Collins S. P.¹^ORCID,McGirr B. A.¹,Liu J.³^ORCID,Beisel C. L.¹⁴⁵^ORCID

Affiliation:

1. Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA.

2. College of Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China.

3. Department of Pharmaceutical Sciences, University of North Texas System College of Pharmacy, University of North Texas Health Science Center, Fort Worth, TX 76129, USA.

4. Helmholtz Institute of RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), 97080 Würzburg, Germany.

5. Faculty of Medicine, University of Würzburg, 97078 Würzburg, Germany.

Abstract

SpyCas9 and its engineered variants can recognize NYGG PAMs, affecting their use for genome editing and off-target predictions.

Funder

National Science Foundation

National Institutes of Health

Camille and Henry Dreyfus Foundation

Agilent Technologies

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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