Discovery and verification of extracellular microRNA biomarkers for diagnostic and prognostic assessment of preeclampsia at triage-Reference-Cited by-同舟云学术

Discovery and verification of extracellular microRNA biomarkers for diagnostic and prognostic assessment of preeclampsia at triage

Published:2023-12-22 Issue:51 Volume:9 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Morey Robert¹^ORCID,Poling Lara¹^ORCID,Srinivasan Srimeenakshi¹,Martinez-King Carolina¹^ORCID,Anyikam Adanna¹,Zhang-Rutledge Kathy¹^ORCID,To Cuong¹,Hakim Abbas¹^ORCID,Mochizuki Marina¹,Verma Kajal¹^ORCID,Mason Antoinette¹,Tran Vy¹,Meads Morgan²^ORCID,Lamale-Smith Leah¹,Roeder Hilary¹,Horii Mariko²^ORCID,Ramos Gladys A.¹^ORCID,DeHoff Peter¹^ORCID,Parast Mana M.²,Pantham Priyadarshini¹,Laurent Louise C.¹^ORCID

Affiliation:

1. Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.

2. Department of Pathology, University of California, San Diego, La Jolla, CA, USA.

Abstract

We report on the identification of extracellular miRNA (ex-miRNA) biomarkers for early diagnosis and prognosis of preeclampsia (PE). Small RNA sequencing of maternal serum prospectively collected from participants undergoing evaluation for suspected PE revealed distinct patterns of ex-miRNA expression among different categories of hypertensive disorders in pregnancy. Applying an iterative machine learning method identified three bivariate miRNA biomarkers ( miR-522-3p/miR-4732-5p , miR-516a-5p/miR-144-3p , and miR-27b-3p/let-7b-5p ) that, when applied serially, distinguished between PE cases of different severity and differentiated cases from controls with a sensitivity of 93%, specificity of 79%, positive predictive value (PPV) of 55%, and negative predictive value (NPV) of 89%. In a small independent validation cohort, these ex-miRNA biomarkers had a sensitivity of 91% and specificity of 57%. Combining these ex-miRNA biomarkers with the established sFlt1:PlGF protein biomarker ratio performed better than either set of biomarkers alone (sensitivity of 89.4%, specificity of 91.3%, PPV of 95.5%, and NPV of 80.8%).

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Link

https://www.science.org/doi/pdf/10.1126/sciadv.adg7545

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