OsCYP706C2 diverts rice strigolactone biosynthesis to a noncanonical pathway branch-Reference-Cited by-同舟云学术

OsCYP706C2 diverts rice strigolactone biosynthesis to a noncanonical pathway branch

Published:2024-08-30 Issue:35 Volume:10 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Li Changsheng¹²^ORCID,Haider Imran¹³⁴^ORCID,Wang Jian You³^ORCID,Quinodoz Pierre⁵^ORCID,Suarez Duran Hernando G.⁶^ORCID,Méndez Lucía Reyes⁵⁷^ORCID,Horber Robin⁵^ORCID,Fiorilli Valentina⁸^ORCID,Votta Cristina⁸^ORCID,Lanfranco Luisa⁸^ORCID,Correia de Lemos Samara M.⁶⁹^ORCID,Jouffroy Lucile¹⁰^ORCID,Moegle Baptiste¹⁰^ORCID,Miesch Laurence¹⁰^ORCID,De Mesmaeker Alain⁵^ORCID,Medema Marnix H.⁶¹¹^ORCID,Al-Babili Salim³^ORCID,Dong Lemeng¹^ORCID,Bouwmeester Harro J.¹^ORCID

Affiliation:

1. Plant Hormone Biology Group, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, Netherlands.

2. Yuelushan Laboratory, Hunan Provincial Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, 410082, Changsha, P. R. China.

3. Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, The BioActives Lab, Thuwal, 23955-6900, Saudi Arabia.

4. Department of Soil, Plant and Food Sciences, Section of Plant Genetics and Breeding, University of Bari Aldo Moro, 70121 Bari, Italy.

5. Syngenta Crop Protection AG, Schaffhauserstrasse 101, CH-4332 Stein, Switzerland.

6. Bioinformatics Group, Wageningen University & Research, 6708 PB Wageningen, Netherlands.

7. Department of Chemistry, University of Basel, St. Johanns-Ring 19, 4056 Basel, Switzerland.

8. Department of Life Sciences and Systems Biology, University of Turin, Viale P.A. Mattioli 25, 10125 Turin, Italy.

9. Plant genomics and transcriptomics group, Institute of Biosciences, Sao Paulo State University, 13506-900 Rio Claro, Brazil.

10. Equipe Synthèse Organique et Phytochimie, Institut de Chimie du CNRS UMR 7177, Université de Strasbourg, Strasbourg, France.

11. Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE, Leiden, Netherlands.

Abstract

Strigolactones exhibit dual functionality as regulators of plant architecture and signaling molecules in the rhizosphere. The important model crop rice exudes a blend of different strigolactones from its roots. Here, we identify the inaugural noncanonical strigolactone, 4-oxo-methyl carlactonoate (4-oxo-MeCLA), in rice root exudate. Comprehensive, cross-species coexpression analysis allowed us to identify a cytochrome P450, OsCYP706C2, and two methyl transferases as candidate enzymes for this noncanonical rice strigolactone biosynthetic pathway. Heterologous expression in yeast and Nicotiana benthamiana indeed demonstrated the role of these enzymes in the biosynthesis of 4-oxo-MeCLA, which, expectedly, is derived from carlactone as substrate. The oscyp706c2 mutants do not exhibit a tillering phenotype but do have delayed mycorrhizal colonization and altered root phenotype. This work sheds light onto the intricate complexity of strigolactone biosynthesis in rice and delineates its role in symbiosis and development.

Publisher

American Association for the Advancement of Science (AAAS)

Link

https://www.science.org/doi/pdf/10.1126/sciadv.adq3942

Reference85 articles.

1. Strigolactone inhibition of shoot branching

2. Inhibition of shoot branching by new terpenoid plant hormones

3. The Decreased apical dominance1/Petunia hybrida CAROTENOID CLEAVAGE DIOXYGENASE8 Gene Affects Branch Production and Plays a Role in Leaf Senescence, Root Growth, and Flower Development

4. Physiological Effects of the Synthetic Strigolactone Analog GR24 on Root System Architecture in Arabidopsis: Another Belowground Role for Strigolactones?

5. Strigolactones as Germination Stimulants for Root Parasitic Plants