Diversification of reprogramming trajectories revealed by parallel single-cell transcriptome and chromatin accessibility sequencing

Author:

Xing Q. R.12ORCID,El Farran C. A.13ORCID,Gautam P.13ORCID,Chuah Y. S.1,Warrier T.13ORCID,Toh C. X. D.1,Kang N. Y.45ORCID,Sugii S.67ORCID,Chang Y. T.48910ORCID,Xu J.311ORCID,Collins J. J.121314ORCID,Daley G. Q.15161718ORCID,Li H.19ORCID,Zhang L. F.2ORCID,Loh Y. H.132021ORCID

Affiliation:

1. Epigenetics and Cell Fates Laboratory, Programme in Stem Cell, Regenerative Medicine and Aging, Institute of Molecular and Cell Biology, A*STAR, Singapore 138673, Singapore.

2. School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore.

3. Department of Biological Sciences, National University of Singapore, Singapore 117558, Singapore.

4. Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, A*STAR, Singapore 138667, Singapore.

5. Department of Creative IT Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.

6. Institute of Bioengineering and Nanotechnology, A*STAR, Singapore 138669, Singapore.

7. Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, Singapore 169857, Singapore.

8. Department of Chemistry, National University of Singapore, Singapore 117543, Singapore.

9. Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, Republic of Korea.

10. Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.

11. Department of Plant Systems Physiology, Institute for Water and Wetland Research, Radboud University, Heyendaalseweg 135, 6525 AJ, Nijmegen, Netherlands.

12. Institute for Medical Engineering and Science, Department of Biological Engineering, and Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

13. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

14. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.

15. Stem Cell Program, Division of Pediatric Hematology and Oncology, Boston Children’s Hospital and Dana-Farber Cancer Institute, Boston, MA 02115, USA.

16. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.

17. Harvard Stem Cell Institute, Cambridge, MA 02138, USA.

18. Manton Center for Orphan Disease Research, Boston, MA 02115, USA.

19. Center for Individualized Medicine, Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, MN 55905, USA.

20. NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 119077, Singapore.

21. Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.

Abstract

Integrative single-cell analysis de-constructs the heterogeneity of human reprogramming and uncovers its stage-specific GRNs.

Funder

NRF Investigatorship Award

Singapore Ministry of Health’s National Medical Research Council

JCO Development Programme Grant

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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