Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter

Author:

Chien Miao-Ping123ORCID,Brinks Daan124ORCID,Testa-Silva Guilherme15ORCID,Tian He1ORCID,Phil Brooks F.1ORCID,Adam Yoav1ORCID,Bloxham William1,Gmeiner Benjamin1ORCID,Kheifets Simon1ORCID,Cohen Adam E.15ORCID

Affiliation:

1. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

2. Department of Molecular Genetics, Erasmus University Medical Center, Rotterdam, Netherlands.

3. Oncode Institute, Utrecht, Netherlands.

4. Department of Imaging Physics, Delft University of Technology, Delft, Netherlands.

5. Howard Hughes Medical Institute, Cambridge, MA 02138, USA.

Abstract

A photoactivated fluorescent voltage sensor protein combines high sensitivity and optical sectioning in tissue.

Funder

National Institutes of Health

Howard Hughes Medical Institute

ASCRS Research Foundation

Williams College

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference49 articles.

1. Voltage imaging to understand connections and functions of neuronal circuits

2. Genetically encoded indicators of neuronal activity

3. Voltage imaging with genetically encoded indicators

4. Dendrites: bug or feature?

5. Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices;Popovic M.;J. Vis. Exp.,2012

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