Sequentially addressable dielectrophoretic array for high-throughput sorting of large-volume biological compartments

Author:

Isozaki A.12ORCID,Nakagawa Y.1ORCID,Loo M. H.1ORCID,Shibata Y.1ORCID,Tanaka N.1,Setyaningrum D. L.1ORCID,Park J.-W.1,Shirasaki Y.3ORCID,Mikami H.1ORCID,Huang D.1,Tsoi H.1ORCID,Riche C. T.4,Ota T.1,Miwa H.1ORCID,Kanda Y.1ORCID,Ito T.15ORCID,Yamada K.6ORCID,Iwata O.6ORCID,Suzuki K.6ORCID,Ohnuki S.7ORCID,Ohya Y.78ORCID,Kato Y.9,Hasunuma T.910ORCID,Matsusaka S.11,Yamagishi M.3ORCID,Yazawa M.12ORCID,Uemura S.3ORCID,Nagasawa K.13,Watarai H.1314,Di Carlo D.4ORCID,Goda K.14515ORCID

Affiliation:

1. Department of Chemistry, Graduate School of Science, University of Tokyo, East Chemistry Building, Room 213, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

2. Kanagawa Institute of Industrial Science and Technology, 3-2-1 Sakado, Takatsu-ku, Kawasaki-shi, Kanagawa 213-0012, Japan.

3. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Faculty of Science Building 1 (East), Room 575, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

4. Department of Bioengineering, Samueli School of Engineering, University of California, Los Angeles, 420 Westwood Plaza, 5121E Engineering V, Los Angeles, CA 90095, USA.

5. Japan Science and Technology Agency, 4-1-8, Honcho, Kawaguchi-shi, Saitama 332-0012, Japan.

6. R&D Department, euglena Co., Ltd., 75-1, Ono-machi, Tsurumi-ku, Yokohama-shi 230-0046, Japan.

7. Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.

8. AIST-UTokyo Advanced Operando-Measurement Technology Open Innovation Laboratory (OPERANDO-OIL), National Institute of Advanced Industrial Science and Technology (AIST), 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8589, Japan.

9. Graduate School of Science, Technology Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.

10. Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.

11. Clinical Research and Regional Innovation, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.

12. Department of Rehabilitation and Regenerative Medicine, Pharmacology, Columbia University, 650 West 168th Street, BB1108, New York, NY 10032, USA.

13. Division of Stem Cell Cellomics, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.

14. Department of Immunology and Stem Cell Biology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8640, Japan.

15. Institute of Technological Sciences, Wuhan University, Hubei 430072, China.

Abstract

High-throughput large-droplet sorting is demonstrated by an array of sequentially addressable dielectrophoretic sources.

Funder

Japan Society for the Promotion of Science

Japan Science and Technology Agency

Kanagawa Academy of Science and Technology

Murata Science Foundation

Council for Science, Technology and Innovation

White Rock Foundation

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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