Acute multidrug delivery via a wearable bioreactor facilitates long-term limb regeneration and functional recovery in adult Xenopus laevis

Author:

Murugan Nirosha J.12ORCID,Vigran Hannah J.12ORCID,Miller Kelsie A.12ORCID,Golding Annie23ORCID,Pham Quang L.23ORCID,Sperry Megan M.14ORCID,Rasmussen-Ivey Cody12,Kane Anna W.124,Kaplan David L.23ORCID,Levin Michael124ORCID

Affiliation:

1. Department of Biology, Tufts University, Medford, MA, USA.

2. Allen Discovery Center at Tufts University, Medford, MA, USA.

3. Department of Biomedical Engineering, Tufts University, Medford, MA, USA.

4. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.

Abstract

Limb regeneration is a frontier in biomedical science. Identifying triggers of innate morphogenetic responses in vivo to induce the growth of healthy patterned tissue would address the needs of millions of patients, from diabetics to victims of trauma. Organisms such as Xenopus laevis —whose limited regenerative capacities in adulthood mirror those of humans—are important models with which to test interventions that can restore form and function. Here, we demonstrate long-term (18 months) regrowth, marked tissue repatterning, and functional restoration of an amputated X. laevis hindlimb following a 24-hour exposure to a multidrug, pro-regenerative treatment delivered by a wearable bioreactor. Regenerated tissues composed of skin, bone, vasculature, and nerves significantly exceeded the complexity and sensorimotor capacities of untreated and control animals’ hypomorphic spikes. RNA sequencing of early tissue buds revealed activation of developmental pathways such as Wnt/β-catenin, TGF-β, hedgehog, and Notch. These data demonstrate the successful “kickstarting” of endogenous regenerative pathways in a vertebrate model.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Cited by 27 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Structure and properties of spider and silkworm silks for tissue engineering and medicine;Silk-Based Biomaterials for Tissue Engineering, Regenerative and Precision Medicine;2024

2. Biophysical control of plasticity and patterning in regeneration and cancer;Cellular and Molecular Life Sciences;2023-12-15

3. Regenerative Engineering of a Limb: From Amputation to Regeneration;Regenerative Engineering and Translational Medicine;2023-11-27

4. Future medicine: from molecular pathways to the collective intelligence of the body;Trends in Molecular Medicine;2023-09

5. Cytoelectric coupling: Electric fields sculpt neural activity and “tune” the brain’s infrastructure;Progress in Neurobiology;2023-07

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3