Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array-Reference-Cited by-同舟云学术

Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array

Published:2019-08-02 Issue:8 Volume:5 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Zhang Yi¹²^ORCID,Minagawa Yoshihiro²,Kizoe Hiroto²^ORCID,Miyazaki Kentaro³⁴^ORCID,Iino Ryota⁵⁶^ORCID,Ueno Hiroshi²^ORCID,Tabata Kazuhito V.²^ORCID,Shimane Yasuhiro¹^ORCID,Noji Hiroyuki²⁷^ORCID

Affiliation:

1. SUGAR Program, X-star, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.

2. Department of Applied Chemistry, School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan.

3. Department of Life Science and Biotechnology, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.

4. Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8561, Japan.

5. Institute for Molecular Science, National Institutes of Natural Sciences, Aichi 444-8787, Japan.

6. SOKENDAI (The Graduate University for Advanced Studies), Kanagawa 240-0193, Japan.

7. Japan Science and Technology Agency, Tokyo 102-0076, Japan.

Abstract

A strategy based on Poisson statistics enables a rapid and efficient acquisition of enzyme mutants from just a few clones.

Funder

Japan Society for the Promotion of Science

Japan Science and Technology Agency

Japan Agency for Marine-Earth Science and Technology

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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