Cryo-EM structure of the HIV-1 Pol polyprotein provides insights into virion maturation

Author:

Harrison Jerry Joe E. K.123ORCID,Passos Dario Oliveira4ORCID,Bruhn Jessica F.45ORCID,Bauman Joseph D.12,Tuberty Lynda12ORCID,DeStefano Jeffrey J.67ORCID,Ruiz Francesc Xavier12ORCID,Lyumkis Dmitry48ORCID,Arnold Eddy12ORCID

Affiliation:

1. Center for Advanced Biotechnology and Medicine (CABM), Piscataway, NJ, USA.

2. Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ, USA.

3. Department of Chemistry, University of Ghana, Legon, Ghana.

4. The Salk Institute for Biological Studies, La Jolla, CA, USA.

5. NanoImaging Services, San Diego, CA, USA.

6. Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, USA.

7. Maryland Pathogen Research Institute, University of Maryland, College Park, MD, USA.

8. Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA.

Abstract

Key proteins of retroviruses and other RNA viruses are translated and subsequently processed from polyprotein precursors by the viral protease (PR). Processing of the HIV Gag-Pol polyprotein yields the HIV structural proteins and enzymes. Structures of the mature enzymes PR, reverse transcriptase (RT), and integrase (IN) aided understanding of catalysis and design of antiretrovirals, but knowledge of the Pol precursor architecture and function before PR cleavage is limited. We developed a system to produce stable HIV-1 Pol and determined its cryo–electron microscopy structure. RT in Pol has a similar arrangement to the mature RT heterodimer, and its dimerization may draw together two PR monomers to activate proteolytic processing. HIV-1 thus may leverage the dimerization interfaces in Pol to regulate assembly and maturation of polyprotein precursors.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3