DNB-based on-chip motif finding: A high-throughput method to profile different types of protein-DNA interactions-Reference-Cited by-同舟云学术

DNB-based on-chip motif finding: A high-throughput method to profile different types of protein-DNA interactions

Published:2020-07-31 Issue:31 Volume:6 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Li Zhuokun¹^ORCID,Wang Xiaojue¹²^ORCID,Xu Dongyang¹^ORCID,Zhang Dengwei¹²^ORCID,Wang Dan¹²³,Dai Xuechen¹²,Wang Qi¹²^ORCID,Li Zhou¹^ORCID,Gu Ying¹^ORCID,Ouyang Wenjie¹^ORCID,Zhao Shuchang¹⁴,Huang Baoqian¹⁵,Gong Jian⁶^ORCID,Zhao Jing¹,Chen Ao¹^ORCID,Shen Yue⁷⁸,Dong Yuliang¹,Zhang Wenwei¹^ORCID,Xu Xun¹²³^ORCID,Xu Chongjun¹⁶⁹^ORCID,Jiang Yuan¹²⁶^ORCID

Affiliation:

1. BGI-Shenzhen, Shenzhen 518083, China.

2. Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, People's Republic of China.

3. Guangdong Provincial Key Laboratory of Genome Read and Write, Shenzhen 518120, China.

4. School of Basic Medicine, Qingdao University, Qingdao 266000, China.

5. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.

6. Complete Genomics Inc., 2904 Orchard Pkwy, San Jose, CA 95134, USA.

7. Guangdong Provincial Key Laboratory of Genome Read and Write, Shenzhen Engineering Laboratory for Innovative Molecular Diagnostics, BGI-ShenzhenShenzhen 518083, China.

8. Guangdong Provincial Academician Workstation of BGI Synthetic Genomics, BGI-Shenzhen, Shenzhen, 518083, China.

9. MGI, BGI-Shenzhen, Shenzhen 518083, China.

Abstract

DocMF uses HT DNB sequencing and sequential imaging to detect the cleavage/binding motifs involved in protein-DNA interactions.

Funder

Shenzhen Municipal Government of China Peacock Plan

the Guangdong Provincial Key Laboratory of Genome Read and Write

Shenzhen Engineering Laboratory for Innovative Molecular Diagnostics

the China National GeneBank

Publisher

American Association for the Advancement of Science (AAAS)

Subject