Plasmid hypermutation using a targeted artificial DNA replisome-Reference-Cited by-同舟云学术

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Plasmid hypermutation using a targeted artificial DNA replisome

Published:2021-07-16 Issue:29 Volume:7 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Yi Xiao¹^ORCID,Khey Joleen²^ORCID,Kazlauskas Romas J.¹³^ORCID,Travisano Michael¹⁴^ORCID

Affiliation:

1. BioTechnology Institute, University of Minnesota, Minneapolis, MN, USA.

2. Department of Plant and Microbial Biology, University of Minnesota, Minneapolis, MN, USA.

3. Department of Biochemistry Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA.

4. Department of Ecology Evolution and Behavior, University of Minnesota, Minneapolis, MN, USA.

Abstract

A designed protein complex processively copies one strand of a plasmid with errors to speed up molecular evolution in vivo.

Funder

Biotechnology Institute, University of Minnesota

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference59 articles.

1. Origin and Evolution of Antibiotic Resistance: The Common Mechanisms of Emergence and Spread in Water Bodies

2. Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

3. Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I

4. An Adaptable Platform for Directed Evolution in Human Cells

5. In vivo continuous evolution of genes and pathways in yeast

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