Rapid generation of maternal mutants via oocyte transgenic expression of CRISPR-Cas9 and sgRNAs in zebrafish-Reference-Cited by-同舟云学术

Rapid generation of maternal mutants via oocyte transgenic expression of CRISPR-Cas9 and sgRNAs in zebrafish

Published:2021-08-06 Issue:32 Volume:7 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Zhang Chong¹^ORCID,Lu Tong¹^ORCID,Zhang Yizhuang¹^ORCID,Li Jiaguang²^ORCID,Tarique Imran¹^ORCID,Wen Fenfen¹^ORCID,Chen Aijun¹^ORCID,Wang Jiasheng¹^ORCID,Zhang Zhuoyu²^ORCID,Zhang Yanjun¹,Shi De-Li³⁴,Shao Ming¹²^ORCID

Affiliation:

1. Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology and Key Laboratory for Experimental Teratology of the Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, China.

2. Shandong University Taishan College, Qingdao 266237, China.

3. Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.

4. Developmental Biology Laboratory, CNRS-UMR7622, Institut de Biologie Paris-Seine, Sorbonne University, Paris 75005, France.

Abstract

A time-saving and deletion-prone method facilitates functional study of maternal factors in zebrafish.

Funder

National Natural Science Foundation of China

the National Key R&D Program of China

Program of Shandong University Qilu Young Scholars

Publisher

American Association for the Advancement of Science (AAAS)

Subject