Affiliation:
1. Department of Neurobiology, Wise Faculty of Life Sciences and Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, Israel.
2. Laboratory of Developmental Genetics, The Rockefeller University, New York, NY, USA.
Abstract
In
Caenorhabditis elegans
worms, epigenetic information transmits transgenerationally. Still, it is unknown whether the effects transfer to the next generation inside or outside of the nucleus. Here, we use the tractability of gene-specific double-stranded RNA–induced silencing to demonstrate that RNA interference can be inherited independently of any nuclear factors via mothers that are genetically engineered to transmit only their ooplasm but not the oocytes’ nuclei to the next generation. We characterize the mechanisms and, using RNA sequencing, chimeric worms, and sequence polymorphism between different isolates, identify endogenous small RNAs which, similarly to exogenous siRNAs, are inherited in a nucleus-independent manner. From a historical perspective, these results might be regarded as partial vindication of discredited cytoplasmic inheritance theories from the 19th century, such as Darwin’s “pangenesis” theory.
Publisher
American Association for the Advancement of Science (AAAS)
Reference77 articles.
1. W. N. Parker The Germ-Plasm: A Theory of Heredity by August Weismann. Translated by W. Newton Parker and Harriet. Rönnfeldt (Charles Scribner 1893).
2. From Heredity Theory to Vererbung: The Transmission Problem, 1850-1915
3. K. Rogers The Cell (Britannica Educational Publishing ed. 2014 2014).
4. BROWNE, J. Charles Darwin: the power of place
5. A new perspective on Darwin’s Pangenesis