Reengineering biocatalysts: Computational redesign of chondroitinase ABC improves efficacy and stability-Reference-Cited by-同舟云学术

Reengineering biocatalysts: Computational redesign of chondroitinase ABC improves efficacy and stability

Published:2020-08-21 Issue:34 Volume:6 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Hettiaratchi Marian H.¹^ORCID,O’Meara Matthew J.²^ORCID,O’Meara Teresa R.³^ORCID,Pickering Andrew J.¹^ORCID,Letko-Khait Nitzan¹,Shoichet Molly S.¹⁴⁵^ORCID

Affiliation:

1. Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, ON M5S 3E5, Canada.

2. Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Ave. #2017, Ann Arbor, MI 48109, USA.

3. Department of Microbiology and Immunology, University of Michigan, 1150 W. Medical Center Dr., Ann Arbor, MI 48109 USA.

4. Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St., Toronto, ON M5S 3G9, Canada.

5. Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada.

Abstract

Computational redesign of chondroitinase ABC introduces 37 point mutations to improve enzyme stability and activity.

Funder

Natural Sciences and Engineering Research Council of Canada

Canada First Research Excellence Fund

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference53 articles.

1. Two Distinct Chondroitin Sulfate ABC Lyases

2. Composite active site of chondroitin lyase ABC accepting both epimers of uronic acid

3. Local delivery of stabilized chondroitinase ABC degrades chondroitin sulfate proteoglycans in stroke-injured rat brains

4. Delayed treatment with chondroitinase ABC promotes sensorimotor recovery and plasticity after stroke in aged rats

5. Chondroitinase ABC has a long-lasting effect on chondroitin sulphate glycosaminoglycan content in the injured rat brain;Lin R.;J. Neurochem.,2008

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