In vivo protein turnover rates in varying oxygen tensions nominate MYBBP1A as a mediator of the hyperoxia response-Reference-Cited by-同舟云学术

In vivo protein turnover rates in varying oxygen tensions nominate MYBBP1A as a mediator of the hyperoxia response

Published:2023-12-08 Issue:49 Volume:9 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Chen Xuewen¹²³^ORCID,Haribowo Augustinus G.¹²,Baik Alan H.¹⁴^ORCID,Fossati Andrea¹⁵⁶^ORCID,Stevenson Erica¹⁵⁶,Chen Yiwen R.⁷,Reyes Nabora S.⁸^ORCID,Peng Tien⁸⁹^ORCID,Matthay Michael A.¹⁰¹¹^ORCID,Traglia Michela¹²,Pico Alexander R.¹²^ORCID,Jarosz Daniel F.⁷¹³,Buchwalter Abigail¹⁰¹⁴¹⁵^ORCID,Ghaemmaghami Sina¹⁶¹⁷^ORCID,Swaney Danielle L.¹⁵⁶^ORCID,Jain Isha H.¹²⁹¹⁰^ORCID

Affiliation:

1. Institute of Cardiovascular Disease, Gladstone Institutes, San Francisco, CA, USA.

2. Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA.

3. Biomedical Sciences Graduate Program, University of California San Francisco, San Francisco, CA, USA.

4. Department of Medicine, Division of Cardiology, University of California San Francisco, San Francisco, CA, USA.

5. Quantitative Biosciences Institute (QBI), University of California San Francisco, San Francisco, CA, USA.

6. Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA, USA.

7. Department of Chemical and Systems Biology, Stanford University, Stanford, CA, USA.

8. Department of Medicine and Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California San Francisco, San Francisco, CA, USA.

9. Bakar Aging Research Institute, University of California San Francisco, San Francisco, CA, USA.

10. Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA.

11. Departments of Medicine and Anesthesia, University of California San Francisco, San Francisco, CA, USA.

12. Institute of Data Science and Biotechnology, Gladstone Institutes, San Francisco, CA, USA.

13. Department of Developmental Biology, Stanford University, CA, USA.

14. Department of Physiology, University of California San Francisco, San Francisco, CA, USA.

15. Chan Zuckerberg Biohub, San Francisco, CA, USA.

16. Mass Spectrometry Resource Laboratory, University of Rochester, Rochester, NY, USA.

17. Department of Biology, University of Rochester, Rochester, NY, USA.

Abstract

Oxygen deprivation and excess are both toxic. Thus, the body’s ability to adapt to varying oxygen tensions is critical for survival. While the hypoxia transcriptional response has been well studied, the post-translational effects of oxygen have been underexplored. In this study, we systematically investigate protein turnover rates in mouse heart, lung, and brain under different inhaled oxygen tensions. We find that the lung proteome is the most responsive to varying oxygen tensions. In particular, several extracellular matrix (ECM) proteins are stabilized in the lung under both hypoxia and hyperoxia. Furthermore, we show that complex 1 of the electron transport chain is destabilized in hyperoxia, in accordance with the exacerbation of associated disease models by hyperoxia and rescue by hypoxia. Moreover, we nominate MYBBP1A as a hyperoxia transcriptional regulator, particularly in the context of rRNA homeostasis. Overall, our study highlights the importance of varying oxygen tensions on protein turnover rates and identifies tissue-specific mediators of oxygen-dependent responses.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Link

https://www.science.org/doi/pdf/10.1126/sciadv.adj4884

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