Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion-Reference-Cited by-同舟云学术

Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion

Published:2022-05-13 Issue:19 Volume:8 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Ludolf Fernanda¹^ORCID,Ramos Fernanda F.¹^ORCID,Bagno Flávia F.²³^ORCID,Oliveira-da-Silva João A.¹,Reis Thiago A. R.¹,Christodoulides Myron⁴^ORCID,Vassallo Paula F.⁵^ORCID,Ravetti Cecilia G.⁶,Nobre Vandack¹⁶^ORCID,da Fonseca Flavio G.²³^ORCID,Coelho Eduardo A. F.¹⁷

Affiliation:

1. Programa de Pós-Graduação em Ciências da Saúde, Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte 30.130-100, Minas Gerais, Brazil.

2. Laboratório de Virologia Básica e Aplicada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31.270-901, Minas Gerais, Brazil.

3. Centro de Tecnologia em Vacinas (CT Vacinas), BH-Tec, Universidade Federal de Minas Gerais, Belo Horizonte 30.130-100, Minas Gerais, Brazil.

4. Neisseria Research Group, Molecular Microbiology, School of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton SO16 6YD, UK.

5. Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte 30.130-100, Minas Gerais, Brazil.

6. Departamento de Clínica Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte 30.130-100, Minas Gerais, Brazil.

7. Departamento de Patologia Clínica, COLTEC, Universidade Federal de Minas Gerais, Belo Horizonte 31.270-901, Minas Gerais, Brazil.

Abstract

Serum-based ELISA (enzyme-linked immunosorbent assay) has been widely used to detect anti–severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies. However, to date, no study has investigated patient urine as a biological sample to detect SARS-CoV-2 virus-specific antibodies. An in-house urine-based ELISA was developed using recombinant SARS-CoV-2 nucleocapsid protein. The presence of SARS-CoV-2 antibodies in urine was established, with 94% sensitivity and 100% specificity for the detection of anti–SARS-CoV-2 antibodies with the urine-based ELISA and 88% sensitivity and 100% specificity with a paired serum-based ELISA. The urine-based ELISA that detects anti–SARS-CoV-2 antibodies is a noninvasive method with potential application as a facile COVID-19 immunodiagnostic platform, which can be used to report the extent of exposure at the population level and/or to assess the risk of infection at the individual level.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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