Electrospray sample injection for single-particle imaging with x-ray lasers

Author:

Bielecki Johan12ORCID,Hantke Max F.13ORCID,Daurer Benedikt J.14ORCID,Reddy Hemanth K. N.1,Hasse Dirk1ORCID,Larsson Daniel S. D.1ORCID,Gunn Laura H.1ORCID,Svenda Martin15,Munke Anna1ORCID,Sellberg Jonas A.15ORCID,Flueckiger Leonie6,Pietrini Alberto1ORCID,Nettelblad Carl17ORCID,Lundholm Ida1,Carlsson Gunilla1ORCID,Okamoto Kenta1,Timneanu Nicusor18ORCID,Westphal Daniel1,Kulyk Olena9,Higashiura Akifumi1011ORCID,van der Schot Gijs112ORCID,Loh Ne-Te Duane413ORCID,Wysong Taylor E.14,Bostedt Christoph1516,Gorkhover Tais14,Iwan Bianca1718ORCID,Seibert M. Marvin1,Osipov Timur14,Walter Peter14,Hart Philip14ORCID,Bucher Maximilian15ORCID,Ulmer Anatoli19,Ray Dipanwita14,Carini Gabriella14,Ferguson Ken R.14,Andersson Inger1ORCID,Andreasson Jakob920ORCID,Hajdu Janos19ORCID,Maia Filipe R. N. C.121ORCID

Affiliation:

1. Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.

2. European XFEL GmbH, Holzkoppel 4, 22869 Schenefeld, Germany.

3. Chemistry Research Laboratory, Department of Chemistry, Oxford University, 12 Mansfield Rd, Oxford OX1 3TA, UK.

4. Centre for BioImaging Sciences, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117557, Singapore.

5. Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-10691 Stockholm, Sweden.

6. ARC Centre of Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe University, Melbourne, Victoria 3086, Australia.

7. Division of Scientific Computing, Department of Information Technology, Science for Life Laboratory, Uppsala University, Lägerhyddsvägen 2 (Box 337), SE-751 05 Uppsala, Sweden.

8. Department of Physics and Astronomy, Uppsala University, Box 516, SE-75120 Uppsala, Sweden.

9. Institute of Physics, ELI Beamlines, Academy of Sciences of the Czech Republic, Na Slovance 2, CZ-18221 Prague, Czech Republic.

10. Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan

11. Department of Virology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8551, Japan

12. Cryo-Electron Microscopy, Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CH Utrecht, Netherlands

13. Department of Physics, National University of Singapore, Singapore, Singapore.

14. Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.

15. Chemical Sciences and Engineering Division, Argonne National Laboratory, Argonne, IL 60439, USA.

16. Department of Physics and Astronomy, Northwestern University, Evanston, IL 60208, USA.

17. Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.

18. Institut für Quantenoptik, Leibniz Universität Hannover, Welfengarten 1, 30167 Hannover, Germany.

19. Institut für Optik und Atomare Physik, Technische Universität Berlin, 10623 Berlin, Germany.

20. Condensed Matter Physics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden.

21. NERSC, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Abstract

Electrospray contaminant-free delivery of proteins to x-ray laser heralds the start of single-protein x-ray diffractive imaging.

Funder

U.S. Department of Energy

Wellcome Trust

European Research Council

Volkswagen Foundation

Swedish Foundation for International Cooperation in Research and Higher Education

Swedish Foundation for Strategic Research

Knut och Alice Wallenbergs Stiftelse

Vetenskapsrådet

Horizon 2020

European Regional Development Fund

Röntgen-Ångström Cluster

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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