Genomic imprinting-like monoallelic paternal expression determines sex of channel catfish-Reference-Cited by-同舟云学术

Genomic imprinting-like monoallelic paternal expression determines sex of channel catfish

Published:2022-12-21 Issue:51 Volume:8 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Wang Wenwen¹^ORCID,Yang Yujia¹,Tan Suxu¹^ORCID,Zhou Tao¹²^ORCID,Liu Yang¹^ORCID,Tian Changxu¹,Bao Lisui¹^ORCID,Xing De¹^ORCID,Su Baofeng¹^ORCID,Wang Jinhai¹^ORCID,Zhang Yu¹,Liu Shikai¹^ORCID,Shi Huitong¹^ORCID,Gao Dongya³,Dunham Rex¹^ORCID,Liu Zhanjiang³^ORCID

Affiliation:

1. The Fish Molecular Genetics and Biotechnology Laboratory, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL, USA.

2. Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.

3. Department of Biology, College of Arts and Sciences, Syracuse University, Syracuse, NY, USA.

Abstract

The X and Y chromosomes of channel catfish have the same gene contents. Here, we report allelic hypermethylation of the X chromosome within the sex determination region (SDR). Accordingly, the X-borne hydin-1 gene was silenced, whereas the Y-borne hydin-1 gene was expressed, making monoallelic expression of hydin-1 responsible for sex determination, much like genomic imprinting. Treatment with a methylation inhibitor, 5-aza-dC, erased the epigenetic marks within the SDR and caused sex reversal of genetic females into phenotypic males. After the treatment, hydin-1 and six other genes related to cell cycle control and proliferative growth were up-regulated, while three genes related to female sex differentiation were down-regulated in genetic females, providing additional support for epigenetic sex determination in catfish. This mechanism of sex determination provides insights into the plasticity of genetic sex determination in lower vertebrates and its connection with temperature sex determination where DNA methylation is broadly involved.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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