Genomic imprinting-like monoallelic paternal expression determines sex of channel catfish-Reference-Cited by-同舟云学术

Genomic imprinting-like monoallelic paternal expression determines sex of channel catfish

Published:2022-12-21 Issue:51 Volume:8 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Wang Wenwen¹^ORCID,Yang Yujia¹,Tan Suxu¹^ORCID,Zhou Tao¹²^ORCID,Liu Yang¹^ORCID,Tian Changxu¹,Bao Lisui¹^ORCID,Xing De¹^ORCID,Su Baofeng¹^ORCID,Wang Jinhai¹^ORCID,Zhang Yu¹,Liu Shikai¹^ORCID,Shi Huitong¹^ORCID,Gao Dongya³,Dunham Rex¹^ORCID,Liu Zhanjiang³^ORCID

Affiliation:

1. The Fish Molecular Genetics and Biotechnology Laboratory, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL, USA.

2. Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.

3. Department of Biology, College of Arts and Sciences, Syracuse University, Syracuse, NY, USA.

Abstract

The X and Y chromosomes of channel catfish have the same gene contents. Here, we report allelic hypermethylation of the X chromosome within the sex determination region (SDR). Accordingly, the X-borne hydin-1 gene was silenced, whereas the Y-borne hydin-1 gene was expressed, making monoallelic expression of hydin-1 responsible for sex determination, much like genomic imprinting. Treatment with a methylation inhibitor, 5-aza-dC, erased the epigenetic marks within the SDR and caused sex reversal of genetic females into phenotypic males. After the treatment, hydin-1 and six other genes related to cell cycle control and proliferative growth were up-regulated, while three genes related to female sex differentiation were down-regulated in genetic females, providing additional support for epigenetic sex determination in catfish. This mechanism of sex determination provides insights into the plasticity of genetic sex determination in lower vertebrates and its connection with temperature sex determination where DNA methylation is broadly involved.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference71 articles.

1. The Divergent Genomes of Teleosts

2. Relationships between Vertebrate ZW and XY Sex Chromosome Systems

3. Evolutionary Diversity and Turn-Over of Sex Determination in Teleost Fishes

4. Novel sex-determining genes in fish and sex chromosome evolution

Cited by 10 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. DNA methylation plays a critical role in testicular maintenance, but not in sex determination of male tilapia Oreochromis niloticus;Aquaculture;2025-01

2. 5 mC modification of steroid hormone biosynthesis-related genes orchestrates feminization of channel catfish induced by high-temperature;Environmental Pollution;2024-09

3. Integrated analysis of the gonadal methylome and transcriptome provides new insights into the expression regulation of sex determination and differentiation genes in spotted scat (Scatophagus argus);Aquaculture;2024-08

4. Epigenome editing: A new approach to aquaculture breeding;Reviews in Aquaculture;2024-06-05

5. DNA methylation reprogramming in teleosts;Evolution & Development;2024-05-23