Ultraprecise single-molecule localization microscopy enables in situ distance measurements in intact cells

Author:

Coelho Simao1ORCID,Baek Jongho1ORCID,Graus Matthew S.1,Halstead James M.1,Nicovich Philip R.2,Feher Kristen1,Gandhi Hetvi1ORCID,Gooding J. Justin3ORCID,Gaus Katharina1

Affiliation:

1. EMBL Australia Node in Single Molecule Science, School of Medical Sciences and the ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, New South Wales, Australia.

2. Allen Institute for Brain Science, Seattle, WA, USA.

3. School of Chemistry, Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of New South Wales, Sydney, New South Wales, Australia.

Abstract

Feedback SMLM achieves ultrahigh localization precision and in situ distance measurements on the biological relevant scales.

Funder

Australian Research Council

National Health and Medical Research Council

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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