Pervasive head-to-tail insertions of DNA templates mask desired CRISPR-Cas9–mediated genome editing events-Reference-Cited by-同舟云学术

Pervasive head-to-tail insertions of DNA templates mask desired CRISPR-Cas9–mediated genome editing events

Published:2020-02-14 Issue:7 Volume:6 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Skryabin Boris V.¹^ORCID,Kummerfeld Delf-Magnus¹^ORCID,Gubar Leonid¹,Seeger Birte¹^ORCID,Kaiser Helena¹,Stegemann Anja¹,Roth Johannes²^ORCID,Meuth Sven G.³^ORCID,Pavenstädt Hermann⁴^ORCID,Sherwood Joanna⁵,Pap Thomas⁵,Wedlich-Söldner Roland⁶^ORCID,Sunderkötter Cord⁷^ORCID,Schwartz Yuri B.⁸^ORCID,Brosius Juergen⁹¹⁰,Rozhdestvensky Timofey S.¹^ORCID

Affiliation:

1. Medical Faculty, Core Facility Transgenic Animal and Genetic Engineering Models (TRAM), University of Muenster, Muenster, Germany.

2. Institute of Immunology, University Hospital Muenster, Muenster, Germany.

3. Clinic of Neurology with Institute of Translational Neurology, University Hospital Muenster, Muenster, Germany.

4. Internal Medicine D, University Hospital Muenster, Muenster, Germany.

5. Institute of Experimental Musculoskeletal Medicine (IMM), University Hospital Muenster, Muenster, Germany.

6. Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.

7. Department of Dermatology and Venereology, University Hospital Halle, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.

8. Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden.

9. Institute of Experimental Pathology (ZMBE), University of Muenster, Muenster, Germany.

10. Institutes for Systems Genetics, West China Hospital, Sichuan University, Chengdu 610041, China.

Abstract

Knock-in genome targeting risks: Comprehensive locus analysis is essential for precision chromosome-editing identification.

Funder

Deutsche Forschungsgemeinschaft

Bundesministerium für Bildung und Forschung

Deutsches Zentrum für Herz-Kreislauf-Forschung

IZKF Muenster

Cancerfonden

Strategic Research grant from Medical Faculty, Umeå University

IMF grant of the Medical Faculty of WWU

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference36 articles.

1. Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases

2. A TALE nuclease architecture for efficient genome editing

3. Multiplex Genome Engineering Using CRISPR/Cas Systems

4. RNA-Guided Human Genome Engineering via Cas9

5. Identification of 86 candidates for small non-messenger RNAs from the archaeon Archaeoglobus fulgidus

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