Mapping a functional cancer genome atlas of tumor suppressors in mouse liver using AAV-CRISPR–mediated direct in vivo screening

Author:

Wang Guangchuan12ORCID,Chow Ryan D.123ORCID,Ye Lupeng12ORCID,Guzman Christopher D.1245ORCID,Dai Xiaoyun12,Dong Matthew B.1235,Zhang Feng67ORCID,Sharp Phillip A.89ORCID,Platt Randall J.1011ORCID,Chen Sidi1241213ORCID

Affiliation:

1. Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.

2. Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.

3. MD-PhD Program, Yale University School of Medicine, New Haven, CT 06510, USA.

4. Immunobiology Program, Yale University School of Medicine, New Haven, CT 06510, USA.

5. Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06510, USA.

6. Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard University, 75 Ames Street, Cambridge, MA 02142, USA.

7. Department of Biological Engineering, MIT, Cambridge, MA 02142, USA.

8. Koch Institute for Integrative Cancer Research, MIT, 77 Massachusetts Avenue, Cambridge, MA 02139–4307, USA.

9. Department of Biology, MIT, Cambridge, MA 02139–4307, USA.

10. Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, 4058 Basel, Switzerland.

11. Department of Chemistry, University of Basel, Petersplatz 1, 4003 Basel, Switzerland.

12. Biological and Biomedical Sciences Program, Yale University School of Medicine, West Haven, CT 06516, USA.

13. Comprehensive Cancer Center, Yale University School of Medicine, West Haven, CT 06516, USA.

Abstract

AAV-mediated CRISPR screens empower autochthonous functional cancer genomics in the mouse liver.

Funder

American Association for Cancer Research

National Cancer Institute

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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