A high-affinity human TCR-like antibody detects celiac disease gluten peptide–MHC complexes and inhibits T cell activation

Author:

Frick Rahel12ORCID,Høydahl Lene S.123ORCID,Petersen Jan45ORCID,du Pré M. Fleur13,Kumari Shraddha6,Berntsen Grete6ORCID,Dewan Alisa E.13ORCID,Jeliazkov Jeliazko R.7ORCID,Gunnarsen Kristin S.12ORCID,Frigstad Terje6,Vik Erik S.6,Llerena Carmen4,Lundin Knut E.A.138ORCID,Yaqub Sheraz910ORCID,Jahnsen Jørgen1011ORCID,Gray Jeffrey J.71213ORCID,Rossjohn Jamie4514ORCID,Sollid Ludvig M.13ORCID,Sandlie Inger12,Løset Geir Åge126ORCID

Affiliation:

1. Centre for Immune Regulation and Department of Immunology, University of Oslo and Oslo University Hospital-Rikshospitalet, Oslo, Norway.

2. Centre for Immune Regulation and Department of Biosciences, University of Oslo, Oslo, Norway.

3. KG Jebsen Coeliac Disease Research Centre, University of Oslo, Oslo, Norway.

4. Infection and Immunity Program, Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.

5. Australian Research Council Centre of Excellence for Advanced Molecular Imaging, Monash University, Clayton, Victoria, Australia.

6. Nextera AS, Oslo, Norway.

7. Program in Molecular Biophysics, Johns Hopkins University, Baltimore, MD, USA.

8. Department of Gastroenterology, Oslo University Hospital-Rikshospitalet, Oslo, Norway.

9. Department of Gastrointestinal Surgery, Oslo University Hospital-Rikshospitalet, Oslo, Norway.

10. Institute of Clinical Medicine, University of Oslo, Oslo, Norway.

11. Department of Gastroenterology, Akershus University Hospital, Lørenskog, Norway.

12. Department of Chemical and Biomolecular Engineering and Institute of NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA.

13. Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD, USA.

14. Institute of Infection and Immunity, Cardiff University School of Medicine, Heath Park, Cardiff, UK.

Abstract

A human TCR-like antibody blocks gluten-dependent activation of celiac disease T cells in vitro and in HLA-DQ2.5 humanized mice.

Funder

National Institutes of Health

Stiftelsen Kristian Gerhard Jebsen

Research Computing Center, University of Chicago

Maryland Advanced Research Computing Center

Norges Forskningsråd

South Eastern Norway Regional Health Authority

Helse Sør-Øst RHF

Amaldi Research Center

Publisher

American Association for the Advancement of Science (AAAS)

Subject

General Medicine,Immunology

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