Single-cell immune profiling reveals thymus-seeding populations, T cell commitment, and multilineage development in the human thymus-Reference-Cited by-同舟云学术

Single-cell immune profiling reveals thymus-seeding populations, T cell commitment, and multilineage development in the human thymus

Published:2022-11-18 Issue:77 Volume:7 Page:
ISSN:2470-9468
Container-title:Science Immunology
language:en
Short-container-title:Sci. Immunol.

Author:

Cordes Martijn¹²^ORCID,Canté-Barrett Kirsten¹³^ORCID,van den Akker Erik B.²⁴⁵^ORCID,Moretti Federico A.¹^ORCID,Kiełbasa Szymon M.⁶^ORCID,Vloemans Sandra A.¹,Garcia-Perez Laura¹^ORCID,Teodosio Cristina¹⁷^ORCID,van Dongen Jacques J. M.¹⁷^ORCID,Pike-Overzet Karin¹^ORCID,Reinders Marcel J. T.²⁴^ORCID,Staal Frank J. T.¹³⁸^ORCID

Affiliation:

1. Department of Immunology, Leiden University Medical Center, Leiden, Netherlands.

2. Leiden Computational Biology Center, Leiden University Medical Center, Leiden, Netherlands.

3. Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, Netherlands.

4. Delft Bioinformatics Lab, Delft University of Technology, Delft, Netherlands.

5. Molecular Epidemiology, Leiden University Medical Center, Leiden, Netherlands.

6. Department of Biomedical Data Sciences, Leiden University Medical Center, Leiden, Netherlands.

7. Centro de Investigación del Cáncer-Instituto de Biología Molecular y Celular del Cáncer (CIC-IBMCC, USAL-CSIC-FICUS), Department of Medicine, University of Salamanca, Salamanca, Spain.

8. Department of Pediatrics, Leiden University Medical Center, Leiden, Netherlands.

Abstract

T cell development in the mouse thymus has been studied extensively, but less is known regarding T cell development in the human thymus. We used a combination of single-cell techniques and functional assays to perform deep immune profiling of human T cell development, focusing on the initial stages of prelineage commitment. We identified three thymus-seeding progenitor populations that also have counterparts in the bone marrow. In addition, we found that the human thymus physiologically supports the development of monocytes, dendritic cells, and NK cells, as well as limited development of B cells. These results are an important step toward monitoring and guiding regenerative therapies in patients after hematopoietic stem cell transplantation.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

General Medicine,Immunology

Reference74 articles.

1. New insights on human T cell development by quantitative T cell receptor gene rearrangement studies and gene expression profiling

2. Notch and Wnt signaling in T-lymphocyte development and acute lymphoblastic leukemia

3. Human thymus contains multipotent progenitors with T/B lymphoid, myeloid, and erythroid lineage potential

4. The development of T cells from stem cells in mice and humans

5. Early stages in the development of human T, natural killer and thymic dendritic cells

Cited by 37 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Developmental immunology and role of host defenses in fetal and neonatal susceptibility to infection;Remington and Klein's Infectious Diseases of the Fetus and Newborn Infant;2025

2. Unraveling the spatial organization and development of human thymocytes through integration of spatial transcriptomics and single-cell multi-omics profiling;Nature Communications;2024-09-06

3. Thymic epithelial organoids mediate T-cell development;Development;2024-09-01

4. The Observed T Cell Receptor Space database enables paired-chain repertoire mining, coherence analysis, and language modeling;Cell Reports;2024-09

5. Engineering Mesoscale T Cell Receptor Clustering by Plug‐and‐Play Nanotools;Advanced Materials;2024-08-27