System-Wide Temporal Characterization of the Proteome and Phosphoproteome of Human Embryonic Stem Cell Differentiation

Author:

Rigbolt Kristoffer T. G.1,Prokhorova Tatyana A.1,Akimov Vyacheslav1,Henningsen Jeanette12,Johansen Pia T.3,Kratchmarova Irina1,Kassem Moustapha34,Mann Matthias56,Olsen Jesper V.6,Blagoev Blagoy1

Affiliation:

1. Center for Experimental BioInformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.

2. Centre of Inflammation and Metabolism, University of Copenhagen, Rigshospitalet, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.

3. Molecular Endocrinology Laboratory, Department of Endocrinology, Odense University Hospital and Medical Biotechnology Center, Winslowsparken 25, DK-5000 Odense, Denmark.

4. Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, 11461 Riyadh, Kingdom of Saudi Arabia.

5. Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.

6. Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3b, DK-2200 Copenhagen, Denmark.

Abstract

Dynamic phosphorylation during stem cell differentiation may control recruitment of DNA methyltransferases to silence genes that maintain pluripotency.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Cell Biology,Molecular Biology,Biochemistry

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