Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi-Reference-Cited by-同舟云学术

Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi

Published:2015-09-08 Issue:393 Volume:8 Page:
ISSN:1945-0877
Container-title:Science Signaling
language:en
Short-container-title:Sci. Signal.

Author:

Housden Benjamin E.¹,Valvezan Alexander J.²,Kelley Colleen¹,Sopko Richelle¹,Hu Yanhui¹,Roesel Charles¹,Lin Shuailiang¹,Buckner Michael¹,Tao Rong¹,Yilmazel Bahar¹,Mohr Stephanie E.¹,Manning Brendan D.²,Perrimon Norbert¹³

Affiliation:

1. Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

2. Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115, USA.

3. Howard Hughes Medical Institute, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

Abstract

Combining targeted CRISPR-mediated gene editing with an RNAi-mediated screen identifies candidate drug targets.

Funder

NIH

Department of Defense

National Cancer Institute Cancer Center Support

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Cell Biology,Molecular Biology,Biochemistry

Reference62 articles.

1. The Tuberous Sclerosis Complex

2. Sirolimus for Angiomyolipoma in Tuberous Sclerosis Complex or Lymphangioleiomyomatosis

3. Efficacy and Safety of Sirolimus in Lymphangioleiomyomatosis

4. Everolimus for Subependymal Giant-Cell Astrocytomas in Tuberous Sclerosis

5. Use and Abuse of RNAi to Study Mammalian Gene Function

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