Canonical cellular stress granules are required for arsenite-induced necroptosis mediated by Z-DNA–binding protein 1

Author:

Szczerba Mateusz1ORCID,Johnson Brian1ORCID,Acciai Francesco2ORCID,Gogerty Carolina13ORCID,McCaughan Megan13ORCID,Williams Jacqueline13ORCID,Kibler Karen V.1,Jacobs Bertram L.13ORCID

Affiliation:

1. Biodesign Center for Immunotherapy, Vaccines and Virotherapy, Arizona State University, Tempe, AZ 85281, USA.

2. College of Health Solutions, Arizona State University, Phoenix, AZ 85004, USA.

3. School of Life Sciences, Arizona State University, Tempe, AZ 85281, USA.

Abstract

Cellular stress granules arise in cells subjected to stress and promote cell survival. A cellular protein that localizes to stress granules is Z-DNA–binding protein 1 (ZBP1), which plays a major role in necroptosis, a programmed cell death pathway mediated by the kinase RIPK3. Here, we showed that the stress granule inducer arsenite activated RIPK3-dependent necroptosis. This pathway required ZBP1, which localized to arsenite-induced stress granules. RIPK3 localized to stress granules in the presence of ZBP1, leading to the formation of ZBP1-RIPK3 necrosomes, phosphorylation of the RIPK3 effector MLKL, and execution of necroptosis. Cells that did not form stress granules did not induce necroptosis in response to arsenite. Together, these results show that arsenite induces ZBP1-mediated necroptosis in a manner dependent on stress granule formation.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Cell Biology,Molecular Biology,Biochemistry

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