Fanciful FRET-Reference-Cited by-同舟云学术

Fanciful FRET

Published:2006-04-18 Issue:331 Volume:2006 Page:
ISSN:1525-8882
Container-title:Science's STKE
language:en
Short-container-title:Sci. STKE

Author:

Vogel Steven S.¹,Thaler Christopher¹,Koushik Srinagesh V.¹

Affiliation:

1. National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Laboratory of Molecular Physiology, 5625 Fishers Lane, Bethesda, MD 20892, USA.

Abstract

The validity of experiments based on Förster resonance energy transfer (FRET), an imaging technique widely used to measure protein-protein interactions in living cells, critically depends on the accurate and precise measurement of FRET efficiency. The use of FRET standards to determine FRET efficiency, and a consideration of such factors as how the abundance of FRET acceptors and the stoichiometry of donors and acceptors in a molecular complex can affect measured FRET efficiency, will enhance the usefulness with which FRET experiments can be interpreted.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

General Medicine

Reference53 articles.

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