Disabling Cas9 by an anti-CRISPR DNA mimic-Reference-Cited by-同舟云学术

Disabling Cas9 by an anti-CRISPR DNA mimic

Published:2017-07-07 Issue:7 Volume:3 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Shin Jiyung¹²,Jiang Fuguo²³,Liu Jun-Jie²⁴,Bray Nicolas L.¹²^ORCID,Rauch Benjamin J.⁵,Baik Seung Hyun¹²,Nogales Eva²⁴⁶^ORCID,Bondy-Denomy Joseph⁵,Corn Jacob E.¹²^ORCID,Doudna Jennifer A.¹²³⁴⁶

Affiliation:

1. Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA.

2. Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.

3. California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA.

4. Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

5. Department of Microbiology and Immunology and Quantitative Biosciences Institute, University of California, San Francisco, San Francisco, CA 94158, USA.

6. Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA.

Abstract

Natural inhibitors of Cas9 pretend to be DNA and block target binding, and using them in human cells can reduce off-target events.

Funder

National Institutes of Health

Howard Hughes Medical Institute

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference31 articles.

1. Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system