Somatic cell reprogramming-free generation of genetically modified pigs-Reference-Cited by-同舟云学术

Somatic cell reprogramming-free generation of genetically modified pigs

Published:2016-09-02 Issue:9 Volume:2 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Tanihara Fuminori¹²,Takemoto Tatsuya³,Kitagawa Eri⁴,Rao Shengbin⁴,Do Lanh Thi Kim¹,Onishi Akira⁵⁶,Yamashita Yukiko³,Kosugi Chisato³,Suzuki Hitomi³,Sembon Shoichiro⁶,Suzuki Shunichi⁶,Nakai Michiko⁶,Hashimoto Masakazu⁷,Yasue Akihiro⁸,Matsuhisa Munehide²,Noji Sumihare⁹,Fujimura Tatsuya⁴^ORCID,Fuchimoto Dai-ichiro⁶,Otoi Takeshige¹

Affiliation:

1. Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University, 2272-1 Ishii, Myozai-gun, Tokushima 779-3233, Japan.

2. Diabetes Therapeutics and Research Center, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.

3. Division of Embryology, Fujii Memorial Institute of Medical Sciences, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.

4. Research and Development Center, NH Foods Ltd., 3-3 Midorigahara, Tsukuba, Ibaraki 300-2646, Japan.

5. Laboratory of Animal Reproduction, Department of Animal Science and Resources, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan.

6. Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba, Ibaraki, 305-0901, Japan.

7. Laboratory for Embryogenesis, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.

8. Department of Orthodontics and Dentofacial Orthopedics, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan.

9. Department of Life System, Institute of Technology and Science, Tokushima University, 2-1 Minami-Jyosanjima-cho, Tokushima 770-8506, Japan.

Abstract

A new and highly efficient method for generating mutant pigs by electroporating the CRISPR/Cas9 system into zygotes.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference28 articles.

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2. Genetically modified pigs for biomedical research;Luo Y.;J. Inherit. Metab. Dis.,2012

3. Somatic cell nuclear transfer cloning: Practical applications and current legislation;Niemann H.;Reprod. Domest. Anim.,2012

4. Genome-wide inactivation of porcine endogenous retroviruses (PERVs)

5. New life for pig-to-human transplants;Reardon S.;Nature,2015

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