Holliday junction trap shows how cells use recombination and a junction-guardian role of RecQ helicase

Author:

Xia Jun12345ORCID,Chen Li-Tzu1234,Mei Qian12346,Ma Chien-Hui78ORCID,Halliday Jennifer A.134,Lin Hsin-Yu1234,Magnan David1345ORCID,Pribis John P.12345ORCID,Fitzgerald Devon M.1234ORCID,Hamilton Holly M.12345,Richters Megan1234,Nehring Ralf B.1234,Shen Xi9,Li Lei9,Bates David1345,Hastings P. J.14,Herman Christophe1345,Jayaram Makkuni78,Rosenberg Susan M.12345

Affiliation:

1. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.

2. Department of Biochemistry, Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.

3. Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA.

4. Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA.

5. Graduate Program in Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, Houston, TX 77030, USA.

6. Systems, Synthetic, and Physical Biology Program, Rice University, Houston, TX 77030, USA.

7. Department of Molecular Biosciences, University of Texas, Austin, TX 78712, USA.

8. Institute of Cell and Molecular Biology, University of Texas, Austin, TX 78712, USA.

9. Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Abstract

Freeze-frame synthetic proteins trap DNA reaction intermediates in single live cells, revealing origins of genome instability.

Funder

National Institutes of Health

W. M. Keck Foundation

Cancer Prevention and Research Institute of Texas

National Science Foundation

Robert F Welch Foundation

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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