A BAC-Based Physical Map of the Major Autosomes of Drosophila melanogaster-Reference-Cited by-同舟云学术

A BAC-Based Physical Map of the Major Autosomes of Drosophila melanogaster

Published:2000-03-24 Issue:5461 Volume:287 Page:2271-2274
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Hoskins Roger A.¹,Nelson Catherine R.²,Berman Benjamin P.²,Laverty Todd R.²,George Reed A.¹,Ciesiolka Lisa¹,Naeemuddin Mohammed¹,Arenson Andrew D.³,Durbin James³,David Robert G.³,Tabor Paul E.³,Bailey Michael R.³,DeShazo Denise R.³,Catanese Joseph⁴,Mammoser †‡ Aaron⁴,Osoegawa Kazutoyo⁴,de † Pieter J.,Jong ⁴,Celniker †‡ Susan E.¹,Gibbs Richard A.³,Rubin Gerald M.²,Scherer Steven E.³

Affiliation:

1. Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

2. Berkeley Drosophila Genome Project, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

3. Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX 77030, USA.

4. Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.

Abstract

We constructed a bacterial artificial chromosome (BAC)–based physical map of chromosomes 2 and 3 of Drosophila melanogaster , which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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