A Whole-Genome Assembly of Drosophila-Reference-Cited by-同舟云学术

A Whole-Genome Assembly of Drosophila

Published:2000-03-24 Issue:5461 Volume:287 Page:2196-2204
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Myers Eugene W.¹,Sutton Granger G.¹,Delcher Art L.¹,Dew Ian M.¹,Fasulo Dan P.¹,Flanigan Michael J.¹,Kravitz Saul A.¹,Mobarry Clark M.¹,Reinert Knut H. J.¹,Remington Karin A.¹,Anson Eric L.¹,Bolanos Randall A.¹,Chou Hui-Hsien¹,Jordan Catherine M.¹,Halpern Aaron L.¹,Lonardi Stefano¹,Beasley Ellen M.¹,Brandon Rhonda C.¹,Chen Lin¹,Dunn Patrick J.¹,Lai Zhongwu¹,Liang Yong¹,Nusskern Deborah R.¹,Zhan Ming¹,Zhang Qing¹,Zheng Xiangqun¹,Rubin Gerald M.²,Adams Mark D.¹,Venter J. Craig¹

Affiliation:

1. Celera Genomics, Inc., 45 West Gude Drive, Rockville, MD 20850, USA.

2. Howard Hughes Medical Institute, Berkeley Drosophila Genome Project, University of California, Berkeley, CA 94720, USA.

Abstract

We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portion of the genome. In addition, there are isolated contigs that we believe represent nonrepetitive pockets within the heterochromatin of the centromeres. Comparison with a previously sequenced 2.9- megabase region indicates that sequencing accuracy within nonrepetitive segments is greater than 99.99% without manual curation. As such, this initial reconstruction of the Drosophila sequence should be of substantial value to the scientific community.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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