Cellulose Synthase-Like CslF Genes Mediate the Synthesis of Cell Wall (1,3;1,4)-ß- d -Glucans

Author:

Burton Rachel A.123,Wilson Sarah M.123,Hrmova Maria123,Harvey Andrew J.123,Shirley Neil J.123,Medhurst Anne123,Stone Bruce A.123,Newbigin Edward J.123,Bacic Antony123,Fincher Geoffrey B.123

Affiliation:

1. Australian Centre for Plant Functional Genomics; School of Agriculture, Food, and Wine; University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia.

2. Australian Centre for Plant Functional Genomics, School of Botany, University of Melbourne, Parkville, VIC 3010, Australia.

3. Department of Biochemistry, La Trobe University, Bundoora, VIC 3086, Australia.

Abstract

A characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-β- d -glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-β- d -glucan content in barley grain to a cluster of cellulose synthase–like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis , we detected (1,3;1,4)-β- d -glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-β- d -glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-β- d -glucan biosynthesis.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference28 articles.

1. Crop biotechnology provides an opportunity to develop a sustainable future

2. (1->3),(1->4)- -d-Glucans in the cell walls of the Poales (sensu lato): an immunogold labeling study using a monoclonal antibody

3. A. Bacic, P. J. Harris, B. A. Stone, in The Biochemistry of Plants, vol. 14, J. Priess, Ed. (Academic Press, New York, 1988), pp. 297–371.

4. Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth

5. G. B. Fincher, B. A. Stone, in Encyclopedia of Grain Science, vol. 1, C. W. Wrigley, H. Corke, C. E. Walker, Eds. (Academic Press, New York, 2004), pp. 206–223.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3