Recognition of the Codon-Anticodon Helix by Ribosomal RNA-Reference-Cited by-同舟云学术

Recognition of the Codon-Anticodon Helix by Ribosomal RNA

Published:1999-09-10 Issue:5434 Volume:285 Page:1722-1725
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Yoshizawa Satoko¹,Fourmy Dominique¹,Puglisi Joseph D.¹

Affiliation:

1. Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305–5126, USA.

Abstract

Translational fidelity is established by ribosomal recognition of the codon-anticodon interaction within the aminoacyl–transfer RNA (tRNA) site (A site) of the ribosome. Experiments are presented that reveal possible contacts between 16 S ribosomal RNA and the codon-anticodon complex. N1 methylation of adenine at position 1492 (A1492) and A1493 interfered with A-site tRNA binding. Mutation of A1492 and A1493 to guanine or cytosine also impaired A-site tRNA binding. The deleterious effects of A1492G or A1493G (or both) mutations were compensated by 2′fluorine substitutions in the mRNA codon. The results suggest that the ribosome recognizes the codon-anticodon complex by adenine contacts to the messenger RNA backbone and provide a mechanism for molecular discrimination of correct versus incorrect codon-anticodon pairs.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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