Formation of Actin Stress Fibers and Focal Adhesions Enhanced by Rho-Kinase

Author:

Amano Mutsuki1,Chihara Kazuyasu1,Kimura Kazushi2,Fukata Yuko1,Nakamura Nao1,Matsuura Yoshiharu3,Kaibuchi Kozo1

Affiliation:

1. M. Amano, K. Chihara, Y. Fukata, N. Nakamura, K. Kaibuchi, Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630-01, Japan.

2. K. Kimura, Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630-01, Japan, and Second Department of Anatomy, Kyoto University Faculty of Medicine, Kyoto 606, Japan.

3. Y. Matsuura, Department of Virology II, National Institute of Health, Tokyo 162, Japan.

Abstract

The small guanosine triphosphatase (GTPase) Rho is implicated in the formation of stress fibers and focal adhesions in fibroblasts stimulated by extracellular signals such as lysophosphatidic acid (LPA). Rho-kinase is activated by Rho and may mediate some biological effects of Rho. Microinjection of the catalytic domain of Rho-kinase into serum-starved Swiss 3T3 cells induced the formation of stress fibers and focal adhesions, whereas microinjection of the inactive catalytic domain, the Rho-binding domain, or the pleckstrin-homology domain inhibited the LPA-induced formation of stress fibers and focal adhesions. Thus, Rho-kinase appears to mediate signals from Rho and to induce the formation of stress fibers and focal adhesions.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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